Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparative RNAseq of Mouse Parp3+/+ and Parp3-/- Neural Stem Progenitor Cells (NPSC)

ABSTRACT: Comparative RNAseq of Mouse Parp3+/+ and Parp3-/- Neural Stem Progenitor Cells (NPSC)

PROVIDER: PRJNA577304 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10272866.fastq.gz	Fastqsanger.gz
	SRR10272867.fastq.gz	Fastqsanger.gz
	SRR10272868.fastq.gz	Fastqsanger.gz
	SRR10272869.fastq.gz	Fastqsanger.gz
	SRR10272870.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Secretomics of human pluripotent stem cell derived neural progenitor cells and induced astrocytes.

Project description:This study examines and compares the protein content in conditioned media collected from neural cell types generated from human pluripotent stem cells. Conditioned media was prepared for 48 hours at a final endpoint of differentiation day 12. Both groups are from parental line WTC11 and cultured as a monolayer on matrigel. Both groups contain a transgene cassette for doxycycline-inducible expression of sox9 and nfia. Doxycycline was only included in the iAstro groups, whereas it was omitted in the neural progenitor cell groups.

2022-01-19 | PXD030821 | Pride

Transcriptome analysis of mouse cerebral cortical neural progenitor cells

Project description:Transcriptome analysis of neural progenitor/stem cells is limited by the lack of a reliable method for cell isolation. We have designed a genetic dual reporter strategy that can allow prospective isolation of cortical neural progenitor cells and their neuronal progeny form the same animals. These cells should be a good cell source for comparative global analysis. Cortical neural progenitor cells and their neuronal progeny were purified using a dual reporter strategy. The purified cells were used for comparative expression profiling.

2011-09-19 | E-GEOD-22946 | biostudies-arrayexpress

Mina53 demethylates histone 4 arginine 3 asymmetric di-methylation to regulate neural stem/progenitor cell identity

Project description:Arginine methylation of histones plays a critical role in regulating gene expression. The writers (methyltransferases) and readers of methylarginine marks are well-known, but the erasers－arginine demethylases－remain mysterious. Here we identify Myc-induced nuclear antigen 53 (Mina53), a jumonji C domain containing protein, as an arginine demethylase for removing asymmetric di-methylation at arginine 3 of histone H4 (H4R3me2a). Using photoaffinity capture method, we first identified Mina53 as an interactor of H4R3me2a. Biochemical assays in vitro and in cells characterized the arginine demethylation activity of Mina53. Molecular dynamics simulations provide further atomic-level evidence that Mina53 acts on H4R3me2a. In a transgenic mouse model, specific Mina53 deletion in neural stem/progenitor cells prevented H4R3me2a demethylation at distinct genes clusters, dysregulating genes important for neural stem/progenitor cell proliferation and differentiation, and consequently impairing the cognitive function of mice. Collectively, we identify Mina53 as a bona fide H4R3me2a eraser, expanding the understanding of epigenetic gene regulation.

2024-10-22 | PXD045247 | Pride

Identification of PRMT1 interaction proteins in cancer and neural stem cells

Project description:We have showed that cancer cells (or tumorigenic cells) resemble neural stem/progenitor cells in regulatory network, tumorigenicity and differentiation potential. We have shown PRMT1 is a protein that is upreguated in and promotes vaious cancers. The expression of its gene is localized to embryonic neural cells during vertebrate embryogenesis. The project is to identify the interaction partners of PRMT1, by which PRMT1 regulates neural stemness in both cancer cells and neural stem cells.

2021-12-20 | PXD026851 | Pride

Transcriptome analysis of mouse cerebral cortical neural progenitor cells

2011-09-20 | GSE22946 | GEO

LIN28A interactome analyses in human induced pluripotent stem cell-derived neural progenitor cells

Project description:A single protein can be multifaceted depending on the cellular contexts and interacting molecules. LIN28A is an RNA-binding protein that governs developmental timing, cellular proliferation, differentiation, stem cell pluripotency, and metabolism. In addition to its best-known roles in microRNA biogenesis, diverse molecular roles have been recognized. In the nervous system, LIN28A is known to play critical roles in proliferation and differentiation of neural progenitor cells (NPC). We profiled the endogenous LIN28A-interacting proteins in NPC differentiated from human induced pluripotent stem (iPS) cells using immunoprecipitation and liquid chromatography-tandem mass spectrometry. We identified over 500 LIN28A-interacting proteins, including 156 RNA-independent interactors. Functions of these proteins span a wide range of gene regulatory processes. Our analysis opens multiple avenues for elaborating molecular roles and characteristics of LIN28A.

| MSV000084371 | MassIVE

Long Neural Genes Harbor Recurrent DNA Break Clusters in Neural Stem/Progenitor Cells

Project description:Repair of DNA double-strand breaks (DSBs) by non-homologous end-joining is critical for neural development, and brain cells frequently contain somatic genomic variations that might involve DSB intermediates. We now use an unbiased, high-throughput approach to identify genomic regions harboring recurrent DSBs in primary neural stem/progenitor cells (NSPCs). We identify 27 recurrent DSB clusters (RDCs) and, remarkably, all occur within gene bodies. Most of these NSPC RDCs were detected only upon mild, aphidicolin-induced replication stress, providing a nucleotide-resolution view of replication-associated genomic fragile sites. The vast majority of RDCs occur in long, transcribed, and late-replicating genes. Moreover, almost 90% of identified RDC-containing genes are involved in synapse function and/or neural cell adhesion, with a substantial fraction also implicated in tumor suppression and/or mental disorders. Our characterization of NSPC RDCs reveals a basis of gene fragility and suggests potential impacts of DNA breaks on neurodevelopment and neural functions. We performed high-throughput, genome-wide, translocation sequencing (HTGTS) and GRO-seq in primary mouse neural stem/progenitor cells of the indicated genotypes.

2016-02-15 | E-GEOD-74356 | biostudies-arrayexpress

Comparative genomic hybridization by array of glioblastoma-derived neural stem cells

Project description:Comparative genomic hybridization of glioma neural stem cells and normal human reference DNA

2012-09-21 | E-MTAB-972 | biostudies-arrayexpress

Expression data from neural stem cells treated with endotherial progenitor-derived soluble protein

Project description:Endogenous neural stem cells reside in the mammalian brain during development and within special niche microenvironments during adulthood. We discovered that endothelial progenitor cell-secreted factors promote the self-renewal of adult mouse NSCs in vivo and identified this protein as ECF-L. We also analyzed molecular signalling networks caused by ECF-L in neural stem cells using global gene expression analysis. Neural stem cells were isolated and expanded from adult mouse brain. Neural stem cells were cultured in the presence or absence of ECF-L in the MHM medium. After the designed period of ECF-L treatment, cells were harvested and RNA was extacted, followed by DNA microarray analysis.

2015-04-01 | E-GEOD-57794 | biostudies-arrayexpress

Exosomal miRNAs released by neural stem/progenitor cells versus astrocytes

Project description:Exosomal miRNAs released by neural stem/progenitor cells versus astrocytes

| PRJNA450994 | ENA