Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Saccharomyces cerevisiae S288C

ABSTRACT: CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes

PROVIDER: PRJNA578818 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR10352300.fastq.gz	Fastqsanger.gz
	SRR10352301.fastq.gz	Fastqsanger.gz
	SRR10352302.fastq.gz	Fastqsanger.gz
	SRR10357608.fastq.gz	Fastqsanger.gz
	SRR10357609.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 69

Similar Datasets

Saccharomyces cerevisiae S288C

Project description:A genome-scale CRISPRi library enables comprehensive phenotypic profiling in yeast

| PRJNA579997 | ENA

Saccharomyces cerevisiae S288C genome annotation: a working hypothesis.

Project description:The S. cerevisiae genome is the most well-characterized eukaryotic genome and one of the simplest in terms of identifying open reading frames (ORFs), yet its primary annotation has been updated continually in the decade since its initial release in 1996 (Goffeau et al., 1996). The Saccharomyces Genome Database (SGD; www.yeastgenome.org) (Hirschman et al., 2006), the community-designated repository for this reference genome, strives to ensure that the S. cerevisiae annotation is as accurate and useful as possible. At SGD, the S. cerevisiae genome sequence and annotation are treated as a working hypothesis, which must be repeatedly tested and refined. In this paper, in celebration of the tenth anniversary of the completion of the S. cerevisiae genome sequence, we discuss the ways in which the S. cerevisiae sequence and annotation have changed, consider the multiple sources of experimental and comparative data on which these changes are based, and describe our methods for evaluating, incorporating and documenting these new data.

| S-EPMC3040122 | biostudies-literature

Saccharomyces cerevisiae S288C

Project description:Study of regulatory kinase genetic interaction profiles in different environmental conditions and cellular states

| PRJNA559194 | ENA

Saccharomyces cerevisiae S288C

Project description:Aneuploidy-induced proteotoxic stress can be effectively tolerated without dosage compensation, genetic mutations or stress responses

| PRJNA590310 | ENA

Saccharomyces cerevisiae S288C

Project description:Comprehensive profiling of post-transcriptional regulatory activity

| PRJNA579846 | ENA

Genomic structure of and genome-wide recombination in the Saccharomyces cerevisiae S288C progenitor isolate EM93.

Project description:The diploid isolate EM93 is the main ancestor to the widely used Saccharomyces cerevisiae haploid laboratory strain, S288C. In this study, we generate a high-resolution overview of the genetic differences between EM93 and S288C. We show that EM93 is heterozygous for >45,000 polymorphisms, including large sequence polymorphisms, such as deletions and a Saccharomyces paradoxus introgression. We also find that many large sequence polymorphisms (LSPs) are associated with Ty-elements and sub-telomeric regions. We identified 2,965 genetic markers, which we then used to genotype 120 EM93 tetrads. In addition to deducing the structures of all EM93 chromosomes, we estimate that the average EM93 meiosis produces 144 detectable recombination events, consisting of 87 crossover and 31 non-crossover gene conversion events. Of the 50 polymorphisms showing the highest levels of non-crossover gene conversions, only three deviated from parity, all of which were near heterozygous LSPs. We find that non-telomeric heterozygous LSPs significantly reduce meiotic recombination in adjacent intervals, while sub-telomeric LSPs have no discernable effect on recombination. We identified 203 recombination hotspots, relatively few of which are hot for both non-crossover gene conversions and crossovers. Strikingly, we find that recombination hotspots show limited conservation. Some novel hotspots are found adjacent to heterozygous LSPs that eliminate other hotspots, suggesting that hotspots may appear and disappear relatively rapidly.

| S-EPMC3180460 | biostudies-literature

An inducible CRISPR-interference library for genetic interrogation of Saccharomyces cerevisiae biology

Project description:Genome-scale CRISPR interference (CRISPRi) is widely utilized to study cellular processes in a variety of organisms. To date, a genome-wide CRISPRi library, optimized for targeting the Saccharomyces cerevisiae genome, has not been presented. Here, we have generated a comprehensive, inducible CRISPRi library, based on spacer design rules optimized for yeast. We have validated this library for genome-wide interrogation of gene function across a variety of applications, including accurate discovery of haploinsufficient genes and identification of enzymatic and regulatory genes involved in adenine and arginine biosynthesis. The comprehensive nature of the library also revealed refined spacer design parameters for transcriptional repression, including location, nucleosome occupancy and nucleotide features. CRISPRi screens using this library can identify genes and pathways with high precision and low false discovery rate across a variety of experimental conditions, enabling rapid and reliable genome-wide assessment of genetic function and interactions in S. cerevisiae.

2020-11-27 | GSE159409 | GEO

Saccharomyces cerevisiae strain:S288c, SK1

Project description:Genetic mapping methodology powered by induced genome rearrangements

| PRJDB13506 | ENA

CRISPRi with barcoded expression reporters dissects regulatory networks in human cells

Project description:CRISPRi with barcoded expression reporters dissects regulatory networks in human cells

| PRJNA1154595 | ENA

Saccharomyces cerevisiae

Project description:Kaplan2013: High-Resolution, Quantitative Genetic Analysis of RNA Polymerase II

| PRJNA205623 | ENA