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Transcriptome analysis of dissected tumour tissue from Drosophila melanogaster larval tumour models in CD compared to HSD.

ABSTRACT: Transcriptome analysis of dissected tumour tissue from Drosophila melanogaster larval tumour models in CD compared to HSD.

PROVIDER: PRJNA587732 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10401985_1.fastq.gz	Fastqsanger.gz
	SRR10401985_2.fastq.gz	Fastqsanger.gz
	SRR10401986_1.fastq.gz	Fastqsanger.gz
	SRR10401986_2.fastq.gz	Fastqsanger.gz
	SRR10401987_1.fastq.gz	Fastqsanger.gz

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Transcriptome analysis of dissected tumour tissue from Drosophila melanogaster larval tumour models in CD compared to HSD.

Project description:Transcriptomic profiling of Ras/Src tumours from animals raised on CD versus HSD enabled the identification of (i) candidate tumour-derived factors which may impact muscle-wasting and (ii) candidate amino acid transporters which may impact tumor growth.

2020-07-28 | GSE139957 | GEO

Transcription profiling of Hsd:ICR(CD-1) mouse intestines to study regional variations in ABC transporter expression along the mouse intestinal tract.

Project description:8 week-old male Hsd:ICR(CD-1) mice were fed ad libitum a standard chow (Harlan Teklad diet 2018S) and housed in groups of five. Animals were then divided into 3 pools (n=10) and sacrificed. The small intestine was extracted and divided into three sections, where the first 2-3 cm after the stomach comprised the duodenum, the middle third the jejunum, and the section before the ileo-ceco-colic junction comprised the ileum. Colon was not divided into proximal and distal sections and was treated as a single intestinal section.

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Expression in dissected larval tissues including gut, salivary glands, Malpighian tubules, and the remaining cascade

Project description:Genes expressed in the salivary glands and gut of Hessian fly (Mayetiola destructor) larvae are likely involved in interactions with host plants. Hessian fly larval tissues were derived from three day old larvae that were cultured on susceptible cultivar Newton. Each tissue sample contained 200 individuals dissected from the same stage of insects. The same type of dissected tissues were pooled and total RNA was extracted from development of microarray probes.

2010-06-21 | E-GEOD-18412 | biostudies-arrayexpress

Drosophila Larval Brain Neoplasms Present Tumour-Type Dependent Genome Instability

Project description:Drosophila Larval Brain Neoplasms Present Tumour-Type Dependent Genome Instability

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Transcriptomic profiling in a Drosophila model of diet induced transgenerational epigenetic inheritance.

Project description:The study was conducted to identify differentially expressed genes in 1) whole flies that were fed a high sugar diet (HSD), in comparison to flies treated with control diet (CD), and 2) HSD male line derived F1 and F2 progenies, compared to that derived through CD male line. The ancestral HSD exposure of F1 or F2 flies was thus limited only to F0 males. However, besides comparing F1 and F2 flies raised on CD, the F1 and F2 flies treated with HSD were also compared. F0 HSD females were profiled but not used for the mating purpose.

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High-salt diet inhibits tumour growth in mice via regulating myeloid-derived suppressor cells differentiation

Project description:To investigate the mRNA expression of purified tumour-infiltrating M-MDSCs from the NSD or HSD group, we employed Agilent Whole Genome Oligo Microarrays (one-color) as a discovery platform to identify differentially expressed genes (DEGs) between NSD and HSD group. We identified 5391 differentially expressed genes (DEGs) comprising 2586 upregulated and 2805 downregulated genes with a fold change greater than 1.5 between M-MDSCs from the NSD and HSD groups. Subsequently, the results demonstrated a significant shift towards an antitumour pro-inflammatory phenotype in HSD-fed mice with a dominant increase in NOS2, TNF, IL-12a, Toll-like receptor 4 (TLR4) and chemokine (C-C motif) ligand 19 (CCL19) expression and a decrease in lymphocyte antigen 6 complex, locus C1 (Ly6C1), C-C-C motif chemokine ligand 12 (CXCL12), indoleamine 2,3-dioxygenase 1 (IDO1) and CCL2 expression. Among these differences, the difference in the expression of IL-12, TNF-α, NOS2, IL-10 and arginase 1 (Arg1) was confirmed by ELISA or qRT-PCR . Meanwhile, the 2,586 DEGs upregulated (fold change >1.5) in M-MDSCs from the HSD group were markedly enriched in processes involving cytokine production, cell chemotaxis, INF-γ production, cell maturation, osmotic stress responses, acute inflammatory responses, etc. In addition, Analysis of the microarray dataset indicated that the expression of 2 key TFs (activating transcription factor 2 [ATF2] and NFAT5) responsible for osmotic stress was enhanced in the M-MDSCs from the HSD group . We further found that NFAT5 expression was significantly upregulated in M-MDSCs from the HSD group but that there was no difference in ATF2 expression between the two groups in both tumour models.

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Klebsiella pneumoniae subsp. pneumoniae strain:HSD

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17beta-HSD type 12-like is responsible for maturation-inducing hormone synthesis during oocyte maturation in masu salmon

Project description:The maturation-inducing hormone 17alpha,20beta-dihydroxy-4-pregnen-3-one (DHP) was first identified in the amago salmon. However, although carbonyl reductase-like 20beta-hydroxysteroid dehydrogenase (CR/20beta-HSD) was reported to convert 17alpha-hydroxyprogesterone (17alpha-P) to DHP in rainbow trout, we previously found that CR/20beta-HSD mRNA was not up-regulated in stimulated granulosa cells from masu salmon, which suggests that DHP is synthesized by a different enzyme. Accordingly, the present study aimed to identify the specific 20beta-hydroxysteroid dehydrogenase (20beta-HSD) responsible for DHP production by granulosa cells during final oocyte maturation in masu salmon. Granulosa layers were isolated from ovarian follicles at one month before ovulation and incubated with or without forskolin, which was used to mimic luteinizing hormone. Subsequent RNA-sequencing yielded ~12 million reads, with an average length of 51 bp, and 71,062 contigs of >100 bp were constructed. Of the 953 contigs that were exclusively constructed from the reads of forskolin-induced granulosa layers, tBlastx analysis identified one contig (#f103496) that matched 17beta-hydroxysteroid dehydrogenase type 12. We found that mammalian cells transfected with full-length omhsd17beta12l exhibited considerable 20beta-HSD activity, as indicated by efficient conversion of exogenous 17alpha-P to DHP. In addition, we found that omhsd17beta12l mRNA levels were consistently low in follicles during vitellogenic growth; however, the levels increased significantly during final oocyte maturation. The levels of omhsd17beta12l mRNA were also considerably increased in granulosa layers in which 20beta-HSD activity was induced by salmon pituitary extract. Therefore, we suggest that omhsd17beta12l, not CR/20beta-HSD, is the 20beta-HSD responsible for DHP production by granulosa cells in masu salmon during final oocyte maturation.

2016-08-04 | GSE85134 | GEO

Transcriptome analysis of dissected muscle tissue from whole-animal Drosophila melanogaster tumour models across a time course.

Project description:Transcriptome analysis of dissected muscle tissue from whole-animal Drosophila melanogaster tumour models across a time course.

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Expression profile of third instar larval fat body and midgut tissues

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