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Effect of metformin on mRNA expression in lung cancer cells

ABSTRACT: Effect of metformin on mRNA expression in lung cancer cells

PROVIDER: PRJNA591888 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10534450_1.fastq.gz	Fastqsanger.gz
	SRR10534450_2.fastq.gz	Fastqsanger.gz
	SRR10534451_1.fastq.gz	Fastqsanger.gz
	SRR10534451_2.fastq.gz	Fastqsanger.gz

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Project description:To examine the effect of metformin on lung cancer biology, human lung H226 and H1299 squamous cell carcinoma cell-lines were grown in RPMI-1640 medium with 10% v/v fetal bovine serum and with or without 15 uM metformin hydrochloride for 7-8 days. Medium (with any metformin) was replaced every two days. Paired cultures with and without metformin were grown and maintained in parallel. Three separate paired cultures, all seeded with same stock of frozen cells, were grown.

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