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Arabidopsis Response to Abscisic Acid Regulate RNA NAD+-Capping

ABSTRACT: Arabidopsis Response to Abscisic Acid Regulate RNA NAD+-Capping

PROVIDER: PRJNA596797 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10742200.fastq.gz	Fastqsanger.gz
	SRR10742201.fastq.gz	Fastqsanger.gz
	SRR10742202.fastq.gz	Fastqsanger.gz
	SRR10742203.fastq.gz	Fastqsanger.gz
	SRR10742204.fastq.gz	Fastqsanger.gz

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Project description:Eukaryotic messenger RNAs (mRNAs) possess a 5’-end N7-methyl guanosine (m7G) cap that promotes their translation and stability. However, it was recently demonstrated that eukaryotic mRNAs can also carry a 5' end nicotinamide adenine dinucleotide (NAD+) cap that promotes mRNA decay mediated by the NAD+ decapping enzyme DXO1. However, the dynamic regulation of NAD+ capping in plant remains unknown. Here, we describe the global landscape of NAD+-capped RNAs in Arabidopsis thaliana, and demonstrate that DXO1 is responsible for removal of these 5’-end modifications and facilitates mRNA degradation in plant transcriptomes. We also reveal that in the absence of DXO1 NAD+-capped mRNAs are unstable and processed into smRNAs. Furthermore, we find that Abscisic Acid (ABA) remodel the landscape of RNA cap epitransciptome, and the mRNA lost their NAD+ cap contribute to their stability under ABA. Overall, our results support a link between ABA response and RNA NAD+ capping.

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Arabidopsis Response to Abscisic Acid Regulate RNA NAD+-Capping

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HIV-1 infection affects NAD capping of host cell snRNA and snoRNA

Project description:NAD besides its key role in cellular metabolism can serve as an alternative 5’ cap at several short non-coding RNAs. However, the function of the NAD cap remains elusive. Here, we investigate NAD capping of RNAs upon HIV-1 infection, which is associated with intracellular pellagra – depletion of NAD/NADH cellular pool. We applied NAD captureSeq on HIV-1 infected/noninfected cells and we revealed that four snRNAs (U1, U4ATAC, U5E and U7) and four snoRNAs (snord3G, snord102, snorA50A and snord3B) lost NAD cap upon HIV-1 infection. Interestingly, U1 snRNA was previously shown to be essential for HIV-1 replication. We provide evidence that the NAD cap reduces the stability of the U1 - HIV-1 pre-mRNA duplex. The importance of NAD RNA cap in HIV-1 infection was further supported by NAD decapping enzyme DXO overexpression, which led to increase in HIV-1 infectivity. This is the first example of NAD cap function in mammalian cells and suggests a general role of non-canonical RNA caps in antiviral innate immunity response.

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Landscape of RNA NAD+ capping in Arabidopsis unopened flower bud

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