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Joint Profiling of Chromatin Accessibility and CAR-T Integration Site Analysis at Population and Single-cell Levels

ABSTRACT: Joint Profiling of Chromatin Accessibility and CAR-T Integration Site Analysis at Population and Single-cell Levels

PROVIDER: PRJNA601172 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR10885526_1.fastq.gz	Fastqsanger.gz
	SRR10885526_2.fastq.gz	Fastqsanger.gz
	SRR10885527_1.fastq.gz	Fastqsanger.gz
	SRR10885527_2.fastq.gz	Fastqsanger.gz
	SRR10885528_1.fastq.gz	Fastqsanger.gz

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Joint Profiling of Chromatin Accessibility and CAR-T Integration Site Analysis at Population and Single-cell Levels II

Project description:We report the development of a novel method called EpiVIA (https://github.com/VahediLab/epiVIA) for the joint profiling of the chromatin accessibility and lentiviral integration site analysis at the population and single-cell levels. We validate our technique in clonal cells with previously defined integration sites and further demonstrate the ability to measure lentiviral integration sites and chromatin accessibility of host and viral genomes at the single-cell resolution in CAR-T cells. We anticipate that EpiVIA will enable the single-cell deconstruction of gene regulation during CAR-T therapy, leading to the discovery of cellular factors associated with durable treatment.

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Joint Profiling of Chromatin Accessibility and CAR-T Integration Site Analysis at Population and Single-cell Levels I

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Joint Profiling of Chromatin Accessibility and CAR-T Integration Site Analysis at Population and Single-cell Levels

Project description:This SuperSeries is composed of the SubSeries listed below.

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Joint profiling of chromatin accessibility and CAR-T integration site analysis at population and single-cell levels.

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