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Stagewise keratinocyte differentiation from human embryonic stem cells by defined signal transduction modulators

ABSTRACT: Stagewise keratinocyte differentiation from human embryonic stem cells by defined signal transduction modulators

PROVIDER: PRJNA603109 | ENA |

REPOSITORIES: ENA

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Stagewise keratinocyte differentiation from human embryonic stem cells by defined signal transduction modulators

Project description:we use human embryonic stem cells to demonstrate that epidermal ectoderm and subsequent keratinocyte cell fate can be determined step by step under the regulation of defined factors. We show the global gene expression profiles clustered according to the differentiation stages . We also found the human keratinocyte specific gene expression according to the differentiation stages.

2020-01-25 | GSE144241 | GEO

Stagewise keratinocyte differentiation from human embryonic stem cells by defined signal transduction modulators.

Project description:Keratinocyte is the predominant cell type in the epidermis of skin, and it provides the protective barrier function for the body. Various signaling pathways have been implicated in keratinocyte differentiation in animal models; However, their temporal regulation and interactions are still to be explored in pluripotent stem cell models. In this report, we use human embryonic stem cells to demonstrate that epidermal ectoderm and subsequent keratinocyte cell fate can be determined step by step under the regulation of defined factors. The inhibition of TGF? initiates ectodermal lineage differentiation, and the activation of BMP pathway drives epidermal TP63 expression. Meanwhile, the timely activation of WNT pathway suppresses extraembryonic lineage, and promotes epidermal cell fate. With further specification by NOTCH inhibition, more than 90% of cells become TP63-positive stage ? keratinocytes. Finally, stage ? keratinocytes are produced under defined hypo-calcium keratinocyte culture conditions, and are further matured in mouse xenograft model. This study not only establishes an in vitro platform to study keratinocyte cell fate determination, but also provides an efficient protocol to produce keratinocytes for disease models and clinical applications.

| S-EPMC7085224 | biostudies-literature

Subcellular location defines GPCR signal transduction

Project description:Subcellular location defines GPCR signal transduction

| PRJNA915973 | ENA

RNAi of signal transduction components in HCT116 cells

Project description:RNAi of signal transduction components in HCT116 cells

| PRJEB2536 | ENA

RNAi of signal transduction components in Drosophila S2 cells

Project description:RNAi of signal transduction components in Drosophila S2 cells

| PRJEB2222 | ENA

Two-component signal transduction systems in Streptomyces and related organisms

Project description:Two-component signal transduction systems in Streptomyces and related organisms

| PRJNA110647 | ENA

Hydrogen modulates signal transduction through manipulating oxidized phospholipid mediators

Project description:We have previously demonstrated that molecular hydrogen (H2) neutralizes hydroxyl radical (•OH) in cultured-cells and protects cells and tissues against oxidative stress. Growing evidence has confirmed that H2-consumption exhibits preventive and therapeutic effects in patients with various diseases as well as model animals. Moreover, a small amount of H2 modulates signal transduction for regulating the expression of various genes; however, the primary target of H2 for exhibiting a variety of phenotypes was completely unknown. Here we show that H2 at low amounts manipulates oxidized phospholipid mediators to modulate Ca2+-signal transduction and gene expression.

2014-10-17 | GSE62434 | GEO

Genomic Targets and Features of BarA-UvrY (-SirA) Signal Transduction Systems

Project description:Genomic Targets and Features of BarA-UvrY (-SirA) Signal Transduction Systems

| PRJNA301907 | ENA

Single-cell intracellular epitope and transcript detection revealing signal transduction dynamics

Project description:Single-cell intracellular epitope and transcript detection revealing signal transduction dynamics

| PRJNA681901 | ENA

Expansion of signal transduction pathways in fungi by extensive genome duplication

Project description:Plants and fungi use light and other signals to regulate development, growth, and metabolism. The fruiting bodies of the fungus Phycomyces blakesleeanus are single cells that react to environmental cues, including light, but the underlying mechanisms are largely unknown [1]. The related fungus Mucor circinelloides is an opportunistic human pathogen that changes its mode of growth upon receipt of signals from the environment to facilitate pathogenesis [2]. Understanding how these organisms respond to environmental cues should provide new insights into the mechanisms of sensory perception and signal transduction by a single eukaryotic cell, and their role in pathogenesis. We sequenced the genomes of P. blakesleeanus and M. circinelloides, and show that they have been shaped by an extensive genome duplication or, most likely, a whole genome duplication (WGD), which is rarely observed in fungi [3-6]. We show that the genome duplication has resulted in expansion of gene families, including those involved in signal transduction, and that duplicated genes have specialized, as evidenced by differences in their regulation by light. The transcriptional response to light indeed varies with the developmental stage and is still observed in a photoreceptor mutant of P. blakesleeanus. A phototropic mutant of P. blakesleeanus with a heterozygous mutation in the photoreceptor gene madA demonstrates that photosensor dosage is important for the magnitude of signal transduction. We conclude that the genome duplication provided the means to improve signal transduction for enhanced perception of environmental signals. Our results will help to understand the role of genome dynamics in the evolution of sensory perception in eukaryotes.

2016-04-21 | GSE64369 | GEO

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