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ABSTRACT: Discovery of resistance genes to TSWV

PROVIDER: PRJNA604319 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11006300.fastq.gz	Fastqsanger.gz
	SRR11006301.fastq.gz	Fastqsanger.gz
	SRR11006302.fastq.gz	Fastqsanger.gz
	SRR11006303.fastq.gz	Fastqsanger.gz
	SRR11006304.fastq.gz	Fastqsanger.gz

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RNA-Seq analysis sheds light on the breakdown of Tomato spotted wilt virus resistance in pepper at high temperature

Project description:The Tsw gene conferring Tomato spotted wilt virus (TSWV) resistance in Capsicum chinense breaks down at high temperatures. Here, we performed RNA-Seq analysis under different temperature conditions to uncover the resistance breakdown mechanism using TSWV-resistant C. chinense ‘PI152225’ and susceptible ‘Habanero’. RNA-Seq analysis revealed only a few differentially expressed genes (DEGs) at 1 dpi but 2,096 and 551 DEGs between plants at 25°C and 30°C at 5 dpi. At 5 dpi, there were more upregulated than downregulated DEGs at both temperatures. Gene ontology analysis revealed 30 enriched biological process terms and nine molecular function terms that were enriched among upregulated DEGs at 25°C. We performed KEGG analysis with 805 upregulated DEGs at both temperatures to explore the mechanism underlying resistance breakdown at high temperature. The DEGs were mapped into 10 KEGG pathways. ACC oxidase genes in the ethylene biosynthesis pathway were differentially expressed upon pathogen challenge. Most DEGs from the biological process, molecular function, and cellular components categories involved in resistance responses were downregulated at 30°C. Finally, genes involved in abscisic acid-mediated stress signaling were differentially expressed at high temperature. These results shed light on host–virus interactions and temperature-dependent resistance mechanisms in pepper at the molecular level.

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A TMT-based quantitative proteome analysis to elucidate the TSWV induced signaling cascade in susceptible and resistant cultivars of Solanum lycopersicum

Project description:Tomato spotted wilt virus (TSWV), transmitted by small insects known as thrips, is one of the major threats to tomato productivity across the globe. In addition to tomato, this virus infects more than 1000 other plants belonging to 85 families and is a cause of serious concern. Very little, however, is known about the molecular mechanim of TSWV induced signaling in plants. Here, we used a TMT-based quantitative proteome analysis to investigate the protein profiles of tomato leaves of two cultivars (cv 2621and 2689; susceptible and resistant respectively to TSWV infection) following TSWV inoculation. This approach resulted in the identification of 5112 proteins of which 1022 showed significant changes in response to TSWV. While the proteome of resistant cultivar majorly remain unaltered, proteome of susceptible cultivar showed distint differences following TSWV infection. TSWV modulated proteins in tomato included those with functions previously implicated in plant defence incuding secondary metabolism, ROS detoxification, MAP kinase signaling, Calcium signaling and jasmonate biosynthesis, among others. Taken together, these results provide new insights into the TSWV induced signaling in tomato leaves and may be useful in future to manage this deadly disease of plants.

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