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GSF1635: RNA-seq data of OVCAR3-DAB2IP expressing cells vs. control

ABSTRACT: GSF1635: RNA-seq data of OVCAR3-DAB2IP expressing cells vs. control

PROVIDER: PRJNA605428 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11040750.fastq.gz	Fastqsanger.gz
	SRR11040751.fastq.gz	Fastqsanger.gz
	SRR11040752.fastq.gz	Fastqsanger.gz
	SRR11040753.fastq.gz	Fastqsanger.gz
	SRR11040754.fastq.gz	Fastqsanger.gz

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GSF1635: RNA-seq data of OVCAR3-DAB2IP expressing cells vs. control

Project description:Ovarian cancer (OC) is the leading cause of death from gynecologic malignancies in the US. Ovarian cancer stem cells (OCSCs) have been shown to drive chemoresistance and tumor progression but the mechanism remains incompletely understood. Disabled Homolog 2- Interacting Protein (DAB2IP) is a potent tumor suppressor identified in multiple types of cancers and we demonstrate that DAB2IP is silenced by the repressive epigenetic mark H3K27me3 at the DAB2IP promoter loci in OCSCs.

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lncRNA and mRNA expression signatures in OVCAR3 cells: control vs. miR-200a transfected

Project description:To further development of the effects of miR-200a in ovarian cancer OVCAR3 cells，we have employed lncRNA and mRNA microarray as a discovery platform to identify lncRNA and mRNA expression in miR-200a overexpressing ovarian cancer cells. OVCAR3 cells were transfected with lentiviral vector with eGFP, encoding miR-200a and negative control vector (LV- miR-200a and LV-CON,) by using polybrene. The dysregulation of miR-200a was confirmed by using RT-PCR. RNA was extracted and detected by a lncRNA and mRNA microarray in LV-miR-200a and LV-CON OVCAR3 cells. The different expression of lncRNA and mRNA in LV-miR-200a and LV-CON OVCAR3 cells was analyzed to explore the mechanism that miR-200a affect ovarain cancer cells.

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Effects of DAB2IP expression on gene expression in DLD1 cells

Project description:To assess the function of DAB2IP in colon cancer, we expressed DAB2IP or control LACZ in DLD1 cells which lack DAB2IP.

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1833 cells expressing RKIP vs control

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Effects of DAB2IP depletion on gene expression in HCT116 cells

Project description:To study DAB2IP function on colon cancer, we depleted DAB2IP in HCT116 cells using shDAB2IP or shCNT(scramble) as a control.

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RNASET2-silenced and control OVCAR3 cell clones injected into nude mice.

Project description:To investigate the molecular pathways underlying RNASET2-mediated tumor suppression in vivo, we investigated the gene expression profile of tumor samples obtained from control and RNASET2-silenced OVCAR3 xenografts, respectively. In order to evaluate the contribution of human cancer cells and the murine host stroma to RNASET2-mediated tumorigenesis responses, total RNA extracted from tumor xenografts derived from six independent OVCAR3 cell clones (three parental and three RNASET2-silenced clones) was fluorescently labeled and hybridized to human Agilent whole-genome microarrays. Control and RNASET2-silenced OVCAR3 were injected subcutaneously into nude mice. After 39 days mice were sacrified, tumors were extracted and total RNA purified.

2013-05-01 | E-GEOD-43029 | biostudies-arrayexpress

lncRNA and mRNA expression signatures in OVCAR3 cells: control vs. miR-200a transfected

Project description:lncRNA and mRNA expression signatures in OVCAR3 cells: control vs. miR-200a transfected

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CYGB+ expressing vs non cytoglobin expressing cells (NCE) (MOCK)

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Effects of miR-149-3p overexpression as compared to the effects of DAB2IP depletion.

Project description:PC3 are a metastatic prostate cancer cell line. Microarray analysis was performed to evaluate the impact of miR-149-3p overexpression or DAB2IP depletion in PC3.

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