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ABSTRACT: Nutritional programming in Nile tilapia

PROVIDER: PRJNA607980 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11140804.fastq.gz	Fastqsanger.gz
	SRR11140805.fastq.gz	Fastqsanger.gz
	SRR11140806.fastq.gz	Fastqsanger.gz
	SRR11140807.fastq.gz	Fastqsanger.gz
	SRR11140808.fastq.gz	Fastqsanger.gz

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Project description:Nile Tilapia Nutritional Programming

| PRJNA862496 | ENA

Global DNA methylation changes in Nile tilapia gonad during high-temperature induced masculinization

Project description:In fish, the sex determining mechanisms can broadly be classified as genotypic (GSD), temperature-dependent (TSD), or genotypic plus temperature effects (GSD+TE). For the fish species with TSD or GSD+TE, extremely high or low temperature can affect its sex determination and differentiation. For long time, the underlying changes in DNA methylation that occur during high or low temperature induced sex reversal have not been fully clarified. In this study, we used Nile tilapia as a model to perform a genome-wide survey of differences in DNA methylation in female and male gonads between control and high temperature induced groups using methylated DNA immunoprecipitation (MeDIP). We identified the high temperature induction-related differentially methylated regions (DMRs), and performed functional enrichment analysis for genes exhibiting DMR. These identified differentially methylated genes were potentially involved in the connection between environmental temperature and sex reversal in Nile tilapia. In this study, four samples (control females, CF; control males, CM; induced females, IF; induced males, IM) were analyzed.

2015-08-27 | E-GEOD-72386 | biostudies-arrayexpress

Epigenetic mapping of the somatotropic axis reveals differential DNA hydroxymethylation marks with potential implication in growth

Project description:We report an association of DNA hydroxymethylation profiling at single nucleotide resolution with gene expression in the fast muscle of Nile tilapia.

2021-07-27 | GSE158910 | GEO

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Project description:Nutritional Programming

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Development of gene expression signatures for the HDF and MDA-MB-231 cells that received TP4 (a cationic antimicrobial peptide) treatment

Project description:Triple-negative breast cancer (TNBC) lacks therapeutic target and is difficult to treat. We report a cationic antimicrobial peptide (CAP), tilapia piscidin 4 (TP4), derived from Nile tilapia (Oreochromis niloticus), selectively toxic to TNBC. Here we aim to identify potential target in TNBC cell response to TP4 treatment by microarray study and to further address the role of TP4-resposive genes involved in TNBC cell death.

2016-12-31 | GSE74764 | GEO

Project description:Nile tilapia microbiome

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Project description:Nile tilapia microbiota

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Project description:Nile tilapia and blue tilapia. Variation

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Global DNA methylation changes in Nile tilapia gonad during high-temperature induced masculinization

2015-08-27 | GSE72386 | GEO

Nile tilapia (Oreochromis niloticus) gene expression profiling in males and females at early embryonic development (2, 5 and 9 days post fertilization )

Project description:Commercial production of tilapia relies on monosex cultures of males, which so far proved difficult to maintain in large scale production facilities. Thus, a better understanding of the genetic architecture of the complex trait of sex determination in tilapia is needed.We aimed to detect genes that were differentially expressed by gender at early embryonic development. Artificial fertilization of O. niloticus females with either sex-reversed males (ΔXX) or genetically-modified YY 'supermales' resulted in all-female and all-male embryos, respectively. Pools of all-female and all-male embryos at 2, 5 and 9 days post fertilization were used for custom Agilent eArray. 56 pool samples of Nile tilapia full siblings groups (female or male) at day 2, 5 or 9 post fertilization were subjected to total RNA extraction from whole embryo tissues and hybridized to the custom Agilent array. Each sample was yielded from different cross of artificial fertilization: six dams X five sires. The resulting gender were known based on the sire, sex-reversed males (ΔXX) or genetically-modified YY 'supermales' resulted in all-female and all-male embryos, respectively.

2014-11-01 | E-GEOD-50974 | biostudies-arrayexpress

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