Similar Datasets
Project description:The exchange of mobile genomic islands (MGIs) between microorganisms is often mediated by phages. As a consequence, not only phage genes are transferred, but also genes that have no particular function in the phage's lysogenic cycle. If they provide benefits to the phage's host, such genes are referred to as ‘morons’. The present study was aimed at characterizing a set of Enterobacter cloacae, Klebsiella pneumoniae and Escherichia coli isolates with exceptional antibiotic resistance phenotypes from patients in a neonatal ward. Unexpectedly, these analyses unveiled the existence of a novel family of closely related MGIs in Enterobacteriaceae. The respective MGI from E. cloacae was named MIR17-GI. Importantly, our observations show that MIR17-GI-like MGIs harbor genes associated with high-level resistance to cephalosporins. Further, we show that MIR17-GI-like islands are associated with integrated P4-like prophages. This implicates phages in the spread of cephalosporin resistance amongst Enterobacteriaceae. The discovery of a novel family of MGIs spreading ‘cephalosporinase morons’ is of high clinical relevance, because high-level cephalosporin resistance has serious implications for the treatment of patients with Enterobacteriaceal infections.
2018-08-21 | PXD007113 | Pride
Project description:Retrons are bacterial genetic elements that encode a reverse transcriptase and, in combination with toxic effector proteins, can serve as antiphage defense systems. However, the mechanisms of action of most retron effectors, and how phages evade retrons, are not well understood. Here, we show that some phages can evade retrons and other defense systems by producing specific tRNAs. We find that expression of retron-Eco7 effector proteins (PtuA and PtuB) leads to degradation of tRNA-Tyr and abortive infection. The genomes of T5 phages that evade retron-Eco7 include a tRNA-rich region, including a highly expressed tRNA-Tyr gene, which confers protection against retron-Eco7. Furthermore, we show that other phages (T1, T7) can use a similar strategy, expressing a tRNA-Lys, to counteract a tRNA anticodon defense system (PrrC170).
2024-09-12 | GSE229290 | GEO
Project description:Dual transcriptome of Enterobacter sp. SA187 along with Arabidopsis thaliana root
2019-11-10 | GSE133175 | GEO
Project description:Phage therapy is a therapeutic approach to treat multidrug resistant infections that employs lytic bacteriophages (phages) to eliminate bacteria. Despite the abundant evidence for its success as an antimicrobial in Eastern Europe, there is scarce data regarding its effects on the human host. Here, we aimed to understand how lytic phages interact with cells of the airway epithelium, the tissue site that is colonized by bacterial biofilms in numerous chronic respiratory disorders. Using a panel of Pseudomonas aeruginosa phages and human airway epithelial cells derived from a person with cystic fibrosis, we determined that interactions between phages and epithelial cells depend on specific phage properties as well as physiochemical features of the microenvironment. Although poor at internalizing phages, the airway epithelium responds to phage exposure by changing its transcriptional profile and secreting antiviral and proinflammatory cytokines that correlate with specific phage families. Overall, our findings indicate that mammalian responses to phages are heterogenous and could potentially alter the way that respiratory local defenses aid in bacterial clearance during phage therapy. Thus, besides phage receptor specificity in a particular bacterial isolate, the criteria to select lytic phages for therapy should be expanded to include mammalian cell responses.
2024-02-19 | GSE255619 | GEO
Project description:To investigate the mechanism of PmB resistance and heteroresistance in Enterobacter bugandensis by analysing the differential gene expression under PmB treatment.
2024-05-01 | GSE218614 | GEO
Project description:By performing single-cell RNA sequencing (scRNA-seq) analysis on the T cells which stimulated by phageome from normal or severe acute pancreatitis mice, we seek to understand which T cells contribute to the inflammation in pancreatitis. t-distributed stochastic neighbor embedding (tSNE) identified 5 distinct T cell cluster, including effector CD8, naïve CD8, CD4, proliferating CD8 and stem like CD8. Stimulation by different phages did not change the T cell heterogeneity. Comparison of genes revealed a significant enrichment in the cell proliferation after SAP derived phages treatment. Differential gene expression analysis confirmed that SAP derived phages treatment skewed the cell pattern toward more IL-7r expression, while control derived phages treatment induce the IL-22 expression.
2024-12-31 | GSE269903 | GEO
Project description:Gene content in Salmonella enterica sv Typhimurium TAQ100F2 (Ames strain, cured of Fels phages, and Fels2 reintroduced) after exposure to 2mM hydrogen peroxide Keywords: time-course
2004-05-10 | GSE817 | GEO
Project description:Gene content in Salmonella enterica sv Typhimurium TAQ100F2 (Ames strain, cured of Fels phages and Fels2 reintroduced) 3h after exposure to 2mM hydrogen peroxide Keywords: parallel sample
2004-05-10 | GSE818 | GEO
Project description:Gene content in Salmonella enterica sv Typhimurium TAQ100F1 (Ames strain, cured of Fels phages, and Fels1 reintroduced) 3h after exposure to 2mM hydrogen peroxide Keywords: parallel sample
2004-05-10 | GSE816 | GEO