Project description:Prebiotics can stimulate the growth and activity of probiotics and have a variety of physiological functions. However, the study of walnut oligopeptides as prebiotics to promote probiotics is rarely reported. Therefore, in order to explore the beneficial effect of walnut oligopeptide (WOPs) on Lactobacillus plantarum Z7, WOPs was added to the medium of L. plantarum Z7, and the utilization of WOPs, the effect of WOPs on the biofilm, extracellular polymeric substances, and bacterial death were explored. The results showed that the growth-promoting effect was strengthened with the increasing concentrations of WOPs. The content of bacterial biofilm and EPS increased significantly, and the number of dead bacteria decreased. The beneficial effect of WOPs was probably because that it enhanced the secretion of biofilm which was regulated by bacterial quorum sensing system and promoted the ability of bacteria to resist the adverse environment, thus promoting the growth and reproduction of bacteria.
Project description:To identify the putative salivary tPA activator, we fractionated salivary proteins by size-exclusion chromatography and identified fraction Z8 as the strongest tPA activator, whereas the adjacent fractions Z7 and Z9 activated tPA at lower levels. Mass spectrometry analysis of these fractions identified a total of 152 unique proteins.
