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Microglia coordinate the anti-tumor immune response to breast cancer brain metastasis [MITRG 10X data]

ABSTRACT: Microglia coordinate the anti-tumor immune response to breast cancer brain metastasis [MITRG 10X data]

PROVIDER: PRJNA622587 | ENA |

REPOSITORIES: ENA

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Microglia coordinate the anti-tumor immune response to breast cancer brain metastasis [Foxn1 nu/nu 10X data]

Project description:Microglia coordinate the anti-tumor immune response to breast cancer brain metastasis [Foxn1 nu/nu 10X data]

| PRJNA622588 | ENA

Microglia coordinate the anti-tumor immune response to breast cancer brain metastasis

Project description:Microglia coordinate the anti-tumor immune response to breast cancer brain metastasis

| PRJNA622584 | ENA

Microglia coordinate the anti-tumor immune response to breast cancer brain metastasis [Foxn1 nu/nu 10X data]

Project description:We used single-cell RNA-sequencing to investigate the role of brain-resident microglia versus infiltrating cells in a mouse model of breast cancer brain metastasis (BCBM). We generated brain metastases with a cardiac-injection of the human triple-negative breast cancer cell line MDA-MB-231BR2 (231BR) into Foxn1 nu/nu mice and allowed tumors to grow in the brain for four weeks. We then compared astrocytes (ASCA2+CD45-) and myeloid cells (CD11b+CD45+) from these mice verses control Foxn1 nu/nu brains to find transcriptional changes associated with BCBM.

2023-09-01 | GSE147942 | GEO

Microglia coordinate the anti-tumor immune response to breast cancer brain metastasis [MITRG 10X data]

Project description:We used single-cell RNA-sequencing to investigate the role of myeloid cells in a humanized mouse model of breast cancer brain metastasis (BCBM). We generated brain metastases with a cardiac-injection of the human triple-negative breast cancer cell line MDA-MB-231BR2 (231BR) into MITRG mice and allowed tumors to grow in the brain for 25 days. We then took all human cells (includes myeloid cells and 231BR) from these mice verses control MITRG brains (only myeloid cells) to find transcriptional changes associated with BCBM.

2023-09-01 | GSE147948 | GEO

Microglia coordinate cellular interactions during spinal cord repair

Project description:Microglia coordinate cellular interactions during spinal cord repair

| PRJNA807737 | ENA

Microglia coordinate cellular interactions during spinal cord repair

Project description:Traumatic spinal cord injury (SCI) triggers a neuro-inflammatory response dominated by tissue-resident microglia and monocyte derived macrophages (MDMs). Since activated microglia and MDMs are morphologically identical and express similar phenotypic markers in vivo, identifying injury responses specifically coordinated by microglia has historically been challenging. Here, we pharmacologically depleted microglia and use anatomical, histopathological, tract tracing, bulk and single cell RNA sequencing to reveal the cellular and molecular responses to SCI controlled by microglia. We show that microglia are vital for SCI recovery and coordinate injury responses in CNS-resident glia and infiltrating leukocytes. Depleting microglia exacerbates tissue damage and worsens functional recovery. Conversely, restoring select microglia-dependent signaling axes, identified through sequencing data, in microglia depleted mice prevents secondary damage and promotes recovery. Additional bioinformatics analyses reveal that optimal repair after SCI and likely other forms of neurological disease, might be achieved by co-opting key ligand-receptor interactions between microglia, astrocytes and MDMs.

2022-06-01 | GSE196928 | GEO

Heterogeneity of Brain Metastasis-associated Microglia and Macrophages

Project description:Heterogeneity of Brain Metastasis-associated Microglia and Macrophages

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Proteomic analysis of human microglia cells (HMC3) stimulated with anti-inflammatory cytokines using serum-deprived culture conditions

Project description:The immune functions of microglia in the brain are compromised in the presence of tumors that have the ability to “hijack” their pro-inflammatory nature by sending out signals that promote cancer progression. To analyze the tumor-supportive microglia phenotype, human microglia cells (HMC3) were treated with anti-inflammatory cytokines in serum-deprived cell culture media, and analyzed by LC-MS/MS using an Orbitrap QE instrument.

2021-10-07 | PXD023163 | Pride

Proteomic analysis of human microglia cells (HMC3) stimulated with anti-inflammatory cytokines using serum-enriched culture conditions

Project description:The immune functions of microglia in the brain are compromised in the presence of tumors that have the ability to “hijack” their pro-inflammatory nature by sending out signals that promote cancer progression. To analyze the tumor-supportive microglia phenotype, human microglia cells (HMC3) were treated with anti-inflammatory cytokines in serum-enriched cell culture media, and analyzed by LC-MS/MS using an Orbitrap QE instrument.

2021-10-06 | PXD023166 | Pride

Signaling in microglia after anti-PD-1 treatment

Project description:Signaling in microglia after anti-PD-1 treatment

| PRJNA935332 | ENA