Project description:To investigate the microRNAs involved in the processes of beneficial and detrimental lifestyles, including caloric restriction(CR), exercise and high-fat diet(HF), we performed a comprehensive and thorough comparison of microRNAs expression profiles in liver among these lifestyle modifications.
Project description:The objective of this study was to examine relationships between weight loss through changes in lifestyle and peripheral blood gene expression profiles. Substantial weight loss (-15.2+3.8%) in lifestyle participants was associated with improvement in selected cardiovascular risk factors and significant changes in peripheral blood gene expression from pre- to post-intervention: 132 unique genes showed significant expression changes related to immune function and inflammatory responses involving endothelial activation. In contrast, participants losing minimal weight (-3.1+2.5%) showed only minor changes in cardiovascular risk factors and markers of inflammation, and no changes in gene expression compared to non-intervention controls after 1 year. Weight loss (>10%) during lifestyle modification is associated with down-regulation of genetic pathways governing interactions between circulating immune cells and the vascular endothelium and may be required to successfully reduce CVD risk. A prospective nonrandomized trail was conducted over 1 year in participants undergoing intensive lifestyle modification to reverse or stabilize progression of coronary artery disease. Cardiovascular risk factors, inflammatory biomarkers, and gene expression as a function of weight loss were assessed in 89 lifestyle participants and 71 retrospectively matched controls undergoing usual care.
Project description:The objective of this study was to examine relationships between weight loss through changes in lifestyle and peripheral blood gene expression profiles. Substantial weight loss (-15.2+3.8%) in lifestyle participants was associated with improvement in selected cardiovascular risk factors and significant changes in peripheral blood gene expression from pre- to post-intervention: 132 unique genes showed significant expression changes related to immune function and inflammatory responses involving endothelial activation. In contrast, participants losing minimal weight (-3.1+2.5%) showed only minor changes in cardiovascular risk factors and markers of inflammation, and no changes in gene expression compared to non-intervention controls after 1 year. Weight loss (>10%) during lifestyle modification is associated with down-regulation of genetic pathways governing interactions between circulating immune cells and the vascular endothelium and may be required to successfully reduce CVD risk.
Project description:Epigenome analysis of 18 subjects and 20 controls, before, midpoint, and after a change in diet, exercise, and other lifestyle modifications
Project description:We sought to examine whether urolithin A could alleviate the alcohol-related liver disease. RNA-sequencing (RNA-seq) on mice livers were performed in order to identify the key biological processes and pathways regulated by urolithin A.
Project description:We reasoned that when livers grow beyond their normal size, some growth regulating secreted factors will be suppressed and others will be overexpressed. To obtain overgrown livers, we used genetic models involving YAP or MYC overexpression We the identified differentially expressed genes in both models and filtered out secreted factors by bioinformatically defined secreted protein list.