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PAgo-induced DNA interference protects bacteria from invader DNA [smDNA]

ABSTRACT: pAgo-induced DNA interference protects bacteria from invader DNA [smDNA]

PROVIDER: PRJNA625102 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11541049.fastq.gz	Fastqsanger.gz
	SRR11541050.fastq.gz	Fastqsanger.gz
	SRR11541051.fastq.gz	Fastqsanger.gz
	SRR11541052.fastq.gz	Fastqsanger.gz
	SRR11541053.fastq.gz	Fastqsanger.gz

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pAgo-induced DNA interference protects bacteria from invader DNA

Project description:pAgo-induced DNA interference protects bacteria from invader DNA

| PRJNA625099 | ENA

pAgo-induced DNA interference protects bacteria from invader DNA [gDNA]

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pAgo-induced DNA interference protects bacteria from invader DNA [smDNA]

Project description:Members of the conserved Argonaute protein family use small RNA guides to find their mRNA targets to regulate gene expression and suppress mobile genetic elements in eukaryotes. Argonautes are also present in many bacterial and archaeal species. Unlike eukaryotic proteins, several studied prokaryotic Argonautes use small DNA guides to cleave DNA, a process dubbed DNA interference. However, the natural functions and targets of DNA interference are poorly understood and the mechanisms of DNA guide generation and target discrimination remain unknown. Here, we studied the in vivo activities of a bacterial Argonaute nuclease CbAgo and demonstrated that it induces cleavage of multicopy genetic elements, including plasmids, transposons and repetitive chromosomal loci. Generation of small DNA guides employed by CbAgo requires cooperation between its intrinsic endonuclease activity and the cellular double-strand break repair machinery. The mechanism of guide generation ensures that small DNA guides are enriched in sequences that target foreign DNA and endows CbAgo with capacity to eliminate plasmids and fight phage infection. Similar principles may underlie the specificity of self-nonself discrimination by diverse defense systems in prokaryotes.

2020-07-30 | GSE148595 | GEO

pAgo-induced DNA interference protects bacteria from invader DNA [gDNA]

2020-07-30 | GSE148594 | GEO

pAgo-induced DNA interference protects bacteria from invader DNA

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-07-30 | GSE148596 | GEO

DNA bisulfite sequencing of Invader 4 LTRs in Drosophila

Project description:It has been claimed previously that loss of (cytosine-5) DNA methylation in Dnmt2 mutant embryos affected the establishment of silent chromatin at Invader4 LTRs (Phalke et al., 2009). The inability of Dnmt2 mutants to control TE expression after heat shock (GSE40432) raised the possibility that DNA methylation could be required for control of Invader 4 during the heat shock response. We used DNA bisulfite sequencing at Inv4 LTRs that were previously suggested to be methylated (Phalke et al., 2009).

2017-07-28 | GSE101967 | GEO

RNA interference-independent reprogramming of DNA methylation in Arabidopsis

Project description:RNA interference-independent reprogramming of DNA methylation in Arabidopsis

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QSER1 Protects DNA Methylation Valleys from De Novo Methylation

Project description:QSER1 Protects DNA Methylation Valleys from De Novo Methylation

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Meiotic prophase length modulates Tel1-dependent DNA double-strand break interference

Project description:Meiotic prophase length modulates Tel1-dependent DNA double-strand break interference

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The Fork Protection Complex protects long replicons from DNA damage at the cost of genome instability induced by DNA topological stress [ChIP-seq]

Project description:Tof1/Timeless, protects eukaryotic cells from DNA replication stress as part of the Fork Protection Complex (FPC). Tof1 supports rapid DNA replication, fork pausing, and resolution of DNA topological stress. Here, we show that disruption of FPC function through loss of either Tof1 or Mrc1 results in DNA damage in long replicons. Despite increasing DNA damage in long replicons, loss of either Tof1 or Mrc1 concurrently reduces DNA damage in regions prone to damage caused by DNA topological stress, indicating that the rapid replication promoted by the FPC fosters completing DNA replication at the cost of increased vulnerability to DNA topological stress. Supporting this we find that a tof1 mutation that selectively inhibits DNA topological stress resolution increases DNA damage in contexts prone to DNA topological stress. Our data indicates that the FPC balances rapid replication with recruitment of topoisomerase I to resolve the topological stress generated by increased DNA unwinding.

2024-10-21 | GSE239965 | GEO

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