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Gene expression analysis of human pluripotent stem cells maintained on several laminin-E8 scaffolds.

ABSTRACT: Gene expression analysis of human pluripotent stem cells maintained on several laminin-E8 scaffolds.

PROVIDER: PRJNA629795 | ENA |

REPOSITORIES: ENA

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Gene expression analysis of human pluripotent stem cells maintained on several laminin-E8 scaffolds.

Project description:To identify which gene expressions were affected by the different LM-E8 scaffolds, we performed transcriptome analysis using microarrays.

2021-04-21 | GSE149717 | GEO

Transcriptomic signature of treatment with recombinant laminin 511-E8 and acetazolamide in human H69 cholangiocytes

Project description:IgG4-related cholangitis (IRC) is the hepatobiliary manifestation of IgG4-related disease (IgG4-RD), a systemic, B-cell driven, fibroinflammatory disorder. To date, numerous autoantigens have been described including laminin 511-E8 and carbonic anhydrase (CA) enzymes (PMID 30089633, 15647194). Laminin 511-E8 is an extracellular matrix protein that has been shown to upregulate secretory components and aid in the cholangiocyte differentiation of induced pluripotent stem cells (PMID 27103433). Carbonic anhydrase enzymes catalyse the reversible hydration of carbon dioxide, thereby producing bicarbonate and a single proton. In humans there are 14 different CA isoforms with a confined subcellular distribution. All isoforms can be targeted by the pan-CA inhibitor acetazolamide, which is a registered drug for multiple indications. We aimed to investigate the role of IgG4-RD autoantigens laminin 511-E8 and CA enzymes in human cholangiocytes. Transcriptional profiling may provide insights into the underlying molecular mechanisms of these proteins on the cholangiocellular level. Thus, by RNA sequencing the transcriptomic signature of recombinant laminin 511-E8 and acetazolamide treatment was assessed in human H69 cholangiocytes.

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Human embryonic stem cells in E8 and AKIT culture medium

Project description:Analysis of undifferentiated KhES-1 human embryonic stem cells in growth factors-dependent (E8) and -independent (AKIT) culture medium. Results provide insight into genes differentially expressed in pluripotent states maintained by AKIT and E8 culture medium.

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Transcriptomic signature of treatment with recombinant laminin 511-E8 and acetazolamide in human H69 cholangiocytes

Project description:Transcriptomic signature of treatment with recombinant laminin 511-E8 and acetazolamide in human H69 cholangiocytes

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Project description:Obtaining highly purified differentiated cells via directed differentiation from human pluripotent stem cells (hPSCs) is an essential step for the clinical application of hPSCs. Among the various conditions that should be optimized, the precise role and contribution of the extracellular matrix (ECM) during differentiation are relatively unclear. Here, using a short fragment of laminin 411 (LM411-E8), an ECM predominantly expressed in the vascular endothelial basement membrane, we demonstrated that the directed switching of defined ECMs robustly yields highly purified (>95%) endothelial progenitor cells (PSC-EPCs) from hPSCs in integrin-laminin axis and rho signaling pathway-dependent manner.

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2016-07-21 | GSE73229 | GEO

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