Project description:Sesame seeds is an important traditional crop with high oil content and other abundant nutrients which are very beneficial for diet and health of human being. However, the molecular mechanism for metabolite accumulation, especially for oil and phenylpropanoid biosynthesis, is still not very clear in sesame. In this study, the transcriptome profiles of black and white sesame seeds were compared by RNA-sequencing. Transcriptome analysis showed that the expression patterns of genes encoding phenylpropanoid pathway enzymes were different between the two sesame cultivars. Compared with white sesame, most of genes involved in oil biosynthesis were significantly down-regulated in black sesame.
Project description:We report the genome-wide localization of SESAME and found that three SESAME subunits localized in the gene body. Examining the genome-wide localization of SESAME subunits (Sam1, Ser33, Pyk1) and Set1
Project description:Telomeres are organized into a heterochromatin structure and maintenance of silent heterochromatin is required for chromosome stability. How telomere heterochromatin is dynamically regulated in response to stimuli remains unknown. Pyruvate kinase Pyk1 forms a complex, named SESAME (Serine-responsive SAM-containing Metabolic Enzyme complex) to regulate gene expression by phosphorylating histone H3T11 (H3pT11). Here, we identified a novel function of SESAME in regulating telomere heterochromatin structure. SESAME phosphorylates H3T11 at telomeres, which promotes SIR (silent information regulator) complex assembly at telomeres and protects Sir2 from degradation by autophagy. Moreover, SESAMEcatalyzed H3pT11 directly represses autophagy-related gene expression to further prevent autophagy-mediated Sir2 degradation. By promoting H3pT11, serine increases Sir2 protein levels and enhances telomere silencing. Loss of H3pT11 leads to reduced Sir2 and compromised telomere silencing during chronological aging. Together, our study provides insights into dynamic regulation of silent heterochromatin by histone modifications and autophagy in response to cell metabolism and aging.
Project description:Intervention 1: topical using sesame oil in intervention group. Intervention 2: No intervention in control group.;Treatment - Other;topical using sesame oil in intervention group;No intervention in control group
Primary outcome(s): Severity of pain. Timepoint: First, third, fifth, seventh days. Method of measurement: visual analog scale.
Study Design: Randomization: Randomized, Blinding: Not blinded, Placebo: Not used, Assignment: Parallel, Purpose: Treatment.
Project description:Purpose: The goals of this study are to understand transcriptional changes in the roots of drought-tolerant and sensitive sesame genotypes using PEG (Polyethylene glycol) induced osmotic stress.
