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Gene expression in cauliflower curds

ABSTRACT: Gene expression in cauliflower curds

PROVIDER: PRJNA633027 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11794574_1.fastq.gz	Fastqsanger.gz
	SRR11794574_2.fastq.gz	Fastqsanger.gz
	SRR11794575_1.fastq.gz	Fastqsanger.gz
	SRR11794575_2.fastq.gz	Fastqsanger.gz
	SRR11794576_1.fastq.gz	Fastqsanger.gz

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Gene expression in cauliflower curds

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Project description:The transcription factors LEAFY (LFY) and APETALA1 (AP1), together with the AP1 paralog CAULIFLOWER (CAL), control the onset of flower development in a partially redundant manner. This redundancy is thought to be mediated, at least in part, through the regulation of a shared set of target genes. However, whether these genes are independently or cooperatively regulated by LFY and AP1/CAL, is currently unknown. To better understand the regulatory relationship between LFY and AP1/CAL during floral initiation, we monitored the activity of LFY in the absence of AP1/CAL function. We found that the regulation of several known LFY target genes is unaffected by AP1/CAL perturbation, while others appear to require AP1/CAL activity. Furthermore, we obtained evidence that LFY and AP1/CAL control the expression of some genes in an antagonistic manner. Notably, these include key regulators of floral initiation such TERMINAL FLOWER1 (TFL1), which had been previously reported to be directly repressed by both LFY and AP1. We show here that TFL1 expression is suppressed by AP1 but promoted by LFY. We further demonstrate that LFY has an inhibitory effect on flower formation in the absence of AP1/CAL activity. We propose that LFY and AP1/CAL may act as part of an incoherent feed-forward loop to control the establishment of a stable developmental program for the formation of flowers.

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