Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Nicotiana benthamiana

ABSTRACT: Gene editing using NCas9F via Agrobacterium T4SS

PROVIDER: PRJNA634985 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR11846584_1.fastq.gz	Fastqsanger.gz
	SRR11846584_2.fastq.gz	Fastqsanger.gz
	SRR11846585_1.fastq.gz	Fastqsanger.gz
	SRR11846585_2.fastq.gz	Fastqsanger.gz
	SRR11846586_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Nicotiana benthamiana

Project description:Targeted Gene Editing in Plants using CRISPR-Cas9, Single-Stranded DNA Oligonucleotides, and Protoplast Regeneration

| PRJNA667297 | ENA

Nicotiana benthamiana cell pH mitigation

Project description:We assessed the off-target effects of M2 expression on the leaf proteome of Nicotiana benthamiana infiltrated with the bacterial gene vector Agrobacterium tumefaciens. A iTRAQ quantitative proteomics procedure was followed to compare the leaf proteomes of plants agroinfiltrated with either an ‘empty’ vector or an M2-encoding vector. Leaves infiltrated with the empty vector had a low soluble protein content compared to non-infiltrated control leaves, associated with increased levels of stress-related proteins but decreased levels of photosynthesis-associated proteins. M2 expression partly compromised these effects of agroinfiltration to restore soluble protein content in leaf tissue, associated with restored levels of photosynthesis-associated proteins and reduced levels of stress-related proteins in the apoplast. These data illustrate the cell-wide influence of Golgi lumen pH homeostasis on the leaf proteome of N. benthamiana responding to microbial challenge.

2019-12-06 | PXD014316 | Pride

Nicotiana benthamiana

Project description:Raw sequence reads of M1 shoots of regenerated Nicotiana benthamiana plant lines containing PDS gene editing

| PRJNA916096 | ENA

NGS data of Nicotiana benthamiana 16c plants after CRISPR/Cas9 gene editing

Project description:Provided data came from a detailed study on Nicotiana benthamiana 16c plants where we use Tobacco Rattle Virus (TRV) as a molecular switch to change the chromatin state of a reporter gene (P35S::GFP) from an actively transcribed to a transcriptionally silenced state. Our approach enables us to interrogate different chromatin states of the same locus with the same set of CRISPR/Cas9 genome editing reagents and systematically describe the effect of chromatin state on the frequency and type of mutations induced at various Cas9 targets in a huge set of independently edited cells.

2022-05-04 | E-MTAB-10256 | biostudies-arrayexpress

Nicotiana benthamiana strain:Lab strain

Project description:Gene expression profiles in Nicotiana benthamiana agrobacterium infiltration.

| PRJDB18624 | ENA

Identification of potential human PML interacting proteins in Nicotiana benthamiana

Project description:In spite of the crucial tasks fulfilled by the subnuclear structures known as PML nuclear bodies (PML-NBs) in mammalian cells, the presence of PML-NBs in plant cells could not be shown yet. Interestingly, the key organizer of PML-NBs, human promyelocytic leukemia protein (PML), is not targeted to structures resembling PML-NBs when expressed heterologously in plants, but localizes to interchromosomal areas associated with the nucleolus. In order to identify plant proteins interacting with human PML, a GFP-fusion protein was ectopically expressed in Nicotiana benthamiana using Agrobacterium tumefaciens mediated transformation. The bait protein as well as potential interactors were immunoprecipitated by use of magnetic GFP-Trap beads (Chromotek). Precipitated proteins were analyzed by Nano-LC-MS/MS.

2016-10-10 | PXD004254 | Pride

Nicotiana benthamiana

Project description:Nicotiana benthamiana Transcriptome or Gene expression

| PRJNA412101 | ENA

Nicotiana benthamiana

Project description:Nicotiana benthamiana Transcriptome or Gene expression

| PRJNA177886 | ENA

Nicotiana benthamiana

Project description:Nicotiana benthamiana Transcriptome or Gene expression

| PRJNA96799 | ENA

Nicotiana benthamiana

Project description:Nicotiana benthamiana Transcriptome or Gene expression