Project description:ENO2, a bifunctional protein with enolase activity and transcriptional activity, plays a vital role in plant development and responses to environmental stresses. However, only several downstream genes targeted by ENO2 have been reported so far. Our study here identified hundreds of candidates of ENO2 targets with ChIP-seq and our special anti-ENO2 antibody. These results will help us further study the role of ENO2 in plant growth/development and environmental stress responses.
Project description:The individualized treatment of tumors has always been an urgent problem in clinical practice. Organoids-on-a-chip can reflect the heterogeneity of tumors and is a good model for in vitro anticancer drug screening. In this study, surgical specimens of patients with advanced colorectal cancer will be collected for organoid culture and organoids-on-a- chip. Use organoids-on-a-chip to screen tumor chemotherapy drugs, compare the results of patients’ actual medication regimens, and study the guiding role of organoids in the formulation of precise tumor treatment plans. The investigators will compare the response of organoids to drugs in vitro with the patient’s response to actual chemotherapy and targeted drugs and explore the feasibility and accuracy of organoids-on-a-chip based drug screening for advanced colorectal cancer. The project will establish a screening platform for chemotherapeutic drugs and targeted drugs based on colorectal cancer organoids to quickly and accurately formulate personalized treatment plans for clinical patients.