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Transcriptional variation in the yeast Yarrowia lipolytica depending on substrate and pH conditions applied in bioreactor cultivations

ABSTRACT: Transcriptional variation in the yeast Yarrowia lipolytica depending on substrate and pH conditions applied in bioreactor cultivations

PROVIDER: PRJNA636785 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR11908649_1.fastq.gz	Fastqsanger.gz
	SRR11908649_2.fastq.gz	Fastqsanger.gz
	SRR11908650_1.fastq.gz	Fastqsanger.gz
	SRR11908650_2.fastq.gz	Fastqsanger.gz
	SRR11908651_1.fastq.gz	Fastqsanger.gz

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Transcriptional variation in the yeast Yarrowia lipolytica depending on substrate and pH conditions applied in bioreactor cultivations

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Project description:The goal of the study is to use Next generation sequencing (RNA-seq) and 13C based flux analysis to study the underlying regulation of citric acid metabolism in mixed culture fermentation (glucose and glycerl) of Yarrowia lipolytica. We sequenced the RNA from 4 different samples in the mixed culture (glucose and glycerol) under oxygen excess and limited conditions with 2 replicates each . Transcriptional profiles showed that under oxygen limited conditions, due to deficient mitochondrial activity, citric acid is being consumed back after glycerol exhaustion eventhough glucose is present in excess. Transcriptome and fluxome profiles showed that glucose is mainly directed towards the Pentose phosphate pathway in the dual substrate fermentations.

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