Transcriptome defense response of orange fruit Induced with Rhodoturola mucilaginosa enhanced with salicylic acid
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ABSTRACT:
PROVIDER: PRJNA642106 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR12096783_1.fastq.gz | Fastqsanger.gz | |||
| SRR12096783_2.fastq.gz | Fastqsanger.gz | |||
| SRR12096784_1.fastq.gz | Fastqsanger.gz | |||
| SRR12096784_2.fastq.gz | Fastqsanger.gz | |||
| SRR12096785_1.fastq.gz | Fastqsanger.gz |
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Similar Datasets
Project description:Purpose: The goal of this study is to looked at the expression levels of defense genes in orange fruit in response to the application of the antagonist R. mucilaginosa enhanced with SA Methods: Three identical and evenly distributed wounds (5 mm diameter and 3 mm deep) made at the equator of each fruit were inoculated with 30 µL cell suspension of R. mucilaginosa (1×108 cells/mL) + 0.2 mM SA, whiles cell suspension of R. mucilaginosa alone (1×108 cells/mL) was used as a control. Treated fruits were then kept in plastic baskets and covered with film wraps to maintain relative humidity of 95% at storage temperature of 20 ºC. After 72 h of storage the wound tissues were excise and immediately kept in liquid nitrogen and maintained at -80 ºC for the analysis of the RNA. There were three replications per each sample. Results: The results showed that 46 genes were identified as DEGs. Among these 34 were up-regulated and 12 were down regulated, 10 of the RNA-seq these were validated using qRT-PCR. RNA-seq data had a linear relationship withqRT_PCR with a corelation coefficent value of (R2) 0.8388. Conclusion: Our finding showed that the defense genes of orange fruit were up-regulated by the innoculation of R. mucilaginosa enhanced with 0.2 mM SA in the fruit.
2020-06-27 | GSE153334 | GEO
Project description:We provide evidence that heterosis for bacterial defense exists in hybrids crossed between some Arabidopsis accessions. Comparison of transcriptomes between hybrids exhibiting heterosis for disease resistance and their parents after inoculation with Pst DC3000 revealed that several key genes involved in salicylic acid (SA) biosynthesis were significantly up-regulated in hybrids. Consistently, in response to bacterial infection, more SA [both free SA and SA glycoside (SAG)] accumulated in hybrids compared with both parents. In addition, heterosis for bacterial defense was significantly compromised in hybrids of pad4 mutants in which the SA biosynthesis pathway was blocked. Moreover, we further revealed that increased histone H3 acetylation of the key genes involved in the SA biosynthesis pathway correlated with their up-regulated expression in hybrids. Around 30 inoculated leaves from Arabidopsis accessions Col-0 and Sei-0 as well as their reciprocal hybrids Fcs and Fsc, which showed best-parent heterosis for bacterial defense were pooled in each sample for mRNA Seq using HiSeq 2000 sequencing system (Illumina)
2015-06-02 | E-GEOD-62256 | biostudies-arrayexpress
Project description:We provide evidence that heterosis for bacterial defense exists in hybrids crossed between some Arabidopsis accessions. Comparison of transcriptomes between hybrids exhibiting heterosis for disease resistance and their parents after inoculation with Pst DC3000 revealed that several key genes involved in salicylic acid (SA) biosynthesis were significantly up-regulated in hybrids. Consistently, in response to bacterial infection, more SA [both free SA and SA glycoside (SAG)] accumulated in hybrids compared with both parents. In addition, heterosis for bacterial defense was significantly compromised in hybrids of pad4 mutants in which the SA biosynthesis pathway was blocked. Moreover, we further revealed that increased histone H3 acetylation of the key genes involved in the SA biosynthesis pathway correlated with their up-regulated expression in hybrids.
2015-06-02 | GSE62256 | GEO
Project description:The yeast Rhodotorula mucilaginosa shown the capability to degrade patulin by the intracellular enzymes. However, the enzymes which risponsible for the degradation process was unkonown. Transcriptome change in response to mycotoxin patulin was analyzed. The molecular mechanism of Rhodotorula mucilaginosa withstand patulin was revealed.
2018-12-01 | GSE98937 | GEO
Project description:• To dissect how the genes are dynamically and differentially expressed during fruit development in sweet orange, a comprehensive transcriptomic study was performed in a pleiotropic mutant (MT) and its wild type (WT). • The detection of the fruit transcriptomic changes was conducted at five stages of fruit development by deep sequencing; the obtained millions of reliable tags were mapped on orange unigenes and subjected to cluster analysis and functional categorization. Sugar and organic acid contents were determined based on the prediction of differential biological processes. • The global clustering analysis revealed a total of 14 expression patterns for the genes involved in fruit development of sweet orange. More than 94% of the genes showed differential expression during fruit development. Comparative transcripts profiling between WT and MT revealed that between 410 and 634 genes were significantly differentially expressed at the five stages. Functional categorization indicated that TCA cycle, carotenoid biosynthesis, and pentose phosphate pathway (OPP) were among the most regulated pathways. • This study provided a dynamic-view of the transcriptome changes during fruit ripening in sweet orange; the results highlighted a set of molecular processes involved in the formation of the mutation trait in the orange fruits.
2012-06-22 | GSE22505 | GEO
Project description:• To dissect how the genes are dynamically and differentially expressed during fruit development in sweet orange, a comprehensive transcriptomic study was performed in a pleiotropic mutant (MT) and its wild type (WT). • The detection of the fruit transcriptomic changes was conducted at five stages of fruit development by deep sequencing; the obtained millions of reliable tags were mapped on orange unigenes and subjected to cluster analysis and functional categorization. Sugar and organic acid contents were determined based on the prediction of differential biological processes. • The global clustering analysis revealed a total of 14 expression patterns for the genes involved in fruit development of sweet orange. More than 94% of the genes showed differential expression during fruit development. Comparative transcripts profiling between WT and MT revealed that between 410 and 634 genes were significantly differentially expressed at the five stages. Functional categorization indicated that TCA cycle, carotenoid biosynthesis, and pentose phosphate pathway (OPP) were among the most regulated pathways. • This study provided a dynamic-view of the transcriptome changes during fruit ripening in sweet orange; the results highlighted a set of molecular processes involved in the formation of the mutation trait in the orange fruits. Investigate the transcriptome changes during five fruit developmental stages of two sweet orange genotypes
2012-06-22 | E-GEOD-22505 | biostudies-arrayexpress
Project description:Salicylic acid (SA) is a plant defense hormone required for immunity. Arabidopsis NPR1 and NPR3/NPR4 were previously shown to bind SA and proposed as SA receptors. However, unlike NPR1, loss of NPR3/NPR4 does not block SA-induced defense gene expression. Here we report that NPR3/NPR4 function as transcriptional repressors and SA inhibits their activities to promote the expression of key immune regulators. npr4-4D, a newly identified gain-of-function allele that renders NPR4 unable to bind SA, constitutively represses SA-induced immune responses. In contrast, the equivalent mutation in NPR1 abolishes its function in promoting SA-induced defense gene expression. Further analysis revealed that npr4-4D and npr1-1 have additive effect on blocking SA-induced defense gene expression, suggesting that NPR4 and NPR1 function in parallel to regulate SA-induced immune responses. Our study reveals the molecular functions of SA receptors NPR3/NPR4 and uncovers a brand new mechanism of SA perception.
2018-06-08 | GSE110702 | GEO
Project description:Disease resistance is associated with a plant defense response that involves an integrated set of signal transduction pathways. Gene Expression studies were performed using Arabidopsis wild type seedlings treated with and without salicylic acid (SA). In this study we examined pathogenesis related genes are upregulated. We used Affymetrix GeneChip to study the genome wide gene expression with and without induction of defense by treatment with SA
2009-02-24 | GSE14961 | GEO