Expression data from adipocyte differentiation from diabetic adipose-derived stem cells (dADSC) treated with squalene (Sq) and its derivative (HH-Sq)
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PROVIDER: PRJNA642219 | ENA |
REPOSITORIES: ENA
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Project description:Gene expression profiling reveals functional difference between Sq and HH-Sq on differentiation, metabolism, and lipid droplot formation of dADSC Microarray gene expression profiling was conducted for technical replicates of adipocyte induced dADSC (IN), adipocyte induced and treated with Sq (Sq), adipocyte induced and treated with HH-Sq (HH-Sq) for 14 days (1 µM) to identify its effect in the regulation of adipocyte differentiation, metabolism, and lipid droplet accumulation. Technical replicates represent the same hybridization mixture applied to 3 independent arrays.
2020-06-27 | GSE153391 | GEO
Project description:Gene expression profiling reveals a potential role of SQ, Tri-SQ, and Tetra-SQ in stimulating hair growth in dermal papilla cells. Squalene (SQ) was found to exhibit a strong antioxidant and anti-inflammatory activity; however, its biological effects is underestimated because of its insolubility. To overcome this problem, new amphiphilic SQ derivatives were synthesized and were used in this study to check their potential effect in vitro and in vivo on inducing hair growth while exhibiting an anti-aging property.
2022-08-25 | GSE211839 | GEO
Project description:Global gene expression profiling reveals a potential anti-melanoma effect of SQ-diEG in B16F10 cells. Squalene (SQ) was considered as a promising natural agent in anti-cancer treatment due to its strong antioxidant and anti-inflammatory activity; however, its pharmacological value has been largely underestimated because of its poor solubility and bioavailability. To adress this problem, a novel amphiphilic SQ derivative which bearing ethylene glycol oligomers was synthesized and was used as a permeation enhancer in this study to check its potential effect on anti-melanoma using B16F10 cells B16F10 were murine melanoma cells line, treated with 2.5 µM and 40 µM with all samples for 48 h. Microarray gene expression profiling was conducted for two biological replicates
2023-02-06 | GSE224256 | GEO
Project description:Global gene expression profiling reveals a potential anti-melanoma effect of SQ-diEG in B16F10 lung colonization mice model. Squalene (SQ) was considered as a promising natural agent in anti-cancer treatment due to its strong antioxidant and anti-inflammatory activity; however, its pharmacological value has been largely underestimated because of its poor solubility and bioavailability. To adress this problem, a novel amphiphilic SQ derivative which bearing ethylene glycol oligomers was synthesized and was used as a permeation enhancer in this study to check its potential effect on anti-melanoma metastasis using B16F10 lung colonization mice model. Total 40 six years old male C57BL/6J mice were randomly divided into five groups (8 mice per group), including the control "(-)B16F10 injected and water treated"; the model "(+) B16F10 injected and water treated"; the positive control "(+) B16F10 injected and DTIC treated (Dacarbazine, 70 mg/kg/day)"; the SQ-diEG treatment "(+) B16F10 injected and SQ-diEG treated (5 mg/kg/day)"; the SQ-diEG treatment "(+) B16F10 injected and SQ-diEG treated (25 mg/kg/day)". After one week of acclimatization, B16F10 were murine melanoma cells line which intravenously injected (200000 tumor cells in 100 μl PBS, 0.2 ml/mouse) through the tail vein of mice. Mice was orally administrated with Dacarbazine (DTIC, positive control, 70mg/kg), and SQ-diEG (5mg/kg and 25mg/kg) for 20 days. On the last day of administration, mice was sacrificed and its lung was washed and collect for the further RNA extraction. Finally, the mice microarray gene expression profiling was conducted for two biological replicates.
2024-09-18 | GSE263339 | GEO
Project description:Expression data from SQ, TRI-SQ and Tetra-SQ-treated HFDPCs
| PRJNA872210 | ENA
Project description:Transcriptomic analysis comprehensively revealed the metabolic improvement effects of di-SQ on the liver and white adipose tissue in ob/ob mice. Di-SQ demonstrated effects in improving inflammation and lipid metabolism in both the liver and white adipose tissue of ob/ob mice and restored the expression of genes and GO terms associated with mitochondrial respiration and glucose metabolism. The metabolic disorder observed in untreated ob/ob mice was reversed by a 42-day-di-SQ treatment, the di-SQ treated ob/ob mice showed a gene expression pattern in key metabolic pathways and processes that more closely resembles that of wild-type mice.
2024-12-10 | GSE281432 | GEO
Project description:We performed bulk RNA-seq on squalene/M-CSF-treated macrophages to compute the fold change of genes in the squalene/M-CSF compared to M-CSF alone treated macrophages. We then looked at the distribution of the log fold changes in the skin-derived TREM2 (607 genes) and M2-like (709 genes) macrophage scRNA-seq signatures. We found that the TREM2 gene signature was more highly induced by squalene/M-CSF than the M2-like macrophage signature. In these squalene-induced macrophages, we found upregulation of several of the lipid metabolism and pro-inflammatory genes present in the skin-derived TREM2 macrophage gene signature.
2021-11-09 | GSE188363 | GEO
Project description:Background and Purpose: Squalene is the main hydrocarbon present in extra virgin olive oil and it has been reported to have anti-steatotic properties in different animal models. The aims of this study were to investigate its effects on liver transcriptomics in Male C57BL/6J Apoe-deficient mice. Experimental Approaches: Male C57BL/6J Apoe-deficient mice were fed a purified Western diet with or without squalene during 11 weeks and hepatic squalene content was assessed. Hepatic transcriptomic changes were studied and confirmed by RT-qPCR. Key Results: Squalene supplementation increased its hepatic content. The Cyp2b10 and Cyp2c55 gene expressions were significantly up-regulated by the squalene intake in all animal models, with independence of sex, sexual hormones, dietary fat content, genetic background and dose, and were strongly associated with antioxidant defense capacity and with lipid content and composition. Conclusions and Implications: hepatic squalene exerts its activity through overexpression of these cytochromes and their changes in virgin olive oil diets may be due to squalene.
2021-04-30 | GSE145343 | GEO
Project description:The hypothesis that the squalene of olive oil might influence hepatic gene expression in an apoE and sex-dependent ways was tested in mice. Gene expression was analyzed using DNA microarrays in male apoE-deficient mice that received 1 g/kg/day of squalene for 10 weeks.
2016-03-30 | GSE36932 | GEO
Project description:The hypothesis that the squalene of olive oil might influence hepatic gene expression in an apoE and sex-dependent ways was tested in mice. Gene expression was analyzed using DNA microarrays in male apoE-deficient mice that received 1 g/kg/day of squalene for 10 weeks. As initial screening of potential candidate genes involved in a differential response, only genes with remarkably modified expression (signal log2 ratio > 1.5 or < -1.5) were further considered
2016-03-30 | E-GEOD-36932 | biostudies-arrayexpress