Project description:A549 human lung cancer cells were knocked down with either siControl or siADGRF4. Gene expression levels were analyzed by RNA sequencing. ADGRF4 globally altered expression patterns of genes in A549 cells.
Project description:HSPD1 knockdown was established in A549 cells using an shRNA approach. Secretomes from both shHSPD1-A549 and shControl-A549 cells were collected and subjected to label-free comparative proteome analysis using SWATH-MS.
Project description:Lung cancer cell line, A549 cells, was transfected with siPML or siCtrl for 48hr to knockdown PML expression. WDR4 or vector was overexpressed in A549 cells for 48hr. These four sets of cells were then subjected to microarray profiling using the Human OneArray microarray (version HOA6.1, GEO Platform GPL19137) from Phalanx Biotech Group. Comparison of transcriptomes from siPML/siCtrl and W4/vec in A549 cells
Project description:To explore the effect of LIMA1 on gene transactivation induced by p53, we evaluated the changes in gene expression by RNA-seq in LIMA1 knockdown or control A549 cells.
Project description:To investigate the cytotoxic effect of AKR1B1 depletion, we induced stable AKR1B1 knockdown via shRNA under different growth conditions (glucose vs fructose) We then performed gene expression profiling analysis using data obtained from RNA-seq of control (non-targeting shRNA) or shAKR1B1 A549 cells 5 days after shRNA delivery.
Project description:To explore the function of TIGD1 in lung cancer cells, we performed microarray to analyze the expression pattern changes by knockdown of TIGD1 in A549 cells.
Project description:Lung cancer cell line, A549 cells, was transfected with siPML or siCtrl for 48hr to knockdown PML expression. WDR4 or vector was overexpressed in A549 cells for 48hr. These four sets of cells were then subjected to microarray profiling using the Human OneArray microarray (version HOA6.1, GEO Platform GPL19137) from Phalanx Biotech Group.