Ontology highlight
ABSTRACT:
PROVIDER: PRJNA645300 | ENA |
REPOSITORIES: ENA
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Project description:PDGFRb+ Mesenchymal Cells, but Not NG2+ Mural Cells, Contribute to Cardiac Fat
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Project description:We performed lineage tracing studies using various genetic models, and found that cardiac mesenchymal cells (MCs) contribute to CAs in postnatal development and adult homeostasis. We found that PDGFRb+ cells are heterogenous, and demonstrated that PDGFRb+ MCs, but not NG2+ coronary vascular mural cells, are the major source of intramyocardial adipocytes. We performed single cell RNA sequencing to compare the heterogeneity.
2020-07-21 | GSE154776 | GEO
Project description:In this study, we examined if KLF4 and c-MYC (KM)-transduced murine cardiac mesenchymal progenitors (CMPs) played a role in the differentiation into adipocytes in the absence of adipogenic stimulation cocktails. These progenitors exhibited increased expression of adipogenic-related genes, such as C/EBPα, PPARγ, and Fabp4, activation of the PPAR signaling pathway, and formation of cytoplasmic lipid droplets within 10 days. Moreover, these KM genes sharply increased following a reperfusion insult, resulting in increased ectopic fat formation. Thus, understanding CMP adipogenesis will clarify the pathophysiology of ischemic reperfusion injury and myocardial infarction and may pave the way for better treatment strategies. Adult mouse cardiac mesenchymal progenitor cells were treated with OSKM for 0, 4, 6, or 8 days.
2015-09-01 | E-GEOD-70088 | biostudies-arrayexpress
Project description:Meningeal cells contribute to postnatal cortical neurogenesis.
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Project description:Purpose: To identify the differential transcriptional patterns during postnatal cardiac growth, pressure-induced cardiac hypertrophy and adult mouse hearts Results: Our data revealed novel transcriptional patterns during cardiac growth conditions. The results showed that most of the essential genes are regulated by promoter clearance of paused RNA pol II, while de novo recruitment is required for regulation of mostly speclaized genes during cardiac growth. We have identified two major modes of gene regulation durign cardiac hypertrophy and four transcriptional growth patterns. The results are published in January 2013 issue of Journal of Biological Chemistry. Methods: Hearts were extracted from 1-2day old C57 mice, from mice subjecetd to Transaortic coarctation or adult mice. The hearts were sent to Active Motif for RNA polII and H3K9ac- ChIP-Seq.
2013-09-06 | E-GEOD-50637 | biostudies-arrayexpress
Project description:Purpose: To identify the differential TFIIB bindingl patterns during postnatal cardiac growth, pressure-induced cardiac hypertrophy and adult mouse hearts Methods: Hearts were extracted from 1-2day old C57 mice, from mice subjecetd to Transaortic coarctation or adult mice. The hearts were sent to Active Motif for TFIIB- ChIP-Seq. Results: In accordance with previosly published data (Sayed D, et. al. JBC, 2013 Jan 25;288(4):2546-58) with genome wide polII and H3K9ac status during cardiac hypertrophy (GSE50637), Denovo TFIIB recruitment was restricted to speclaized genes with cardiac hypertrophy. Hearts were extracted from 1-2day old C57 mice, from mice subjecetd to Transaortic coarctation or adult mice. The hearts were sent to Active Motif for TFIIB- ChIP-Seq.
2014-11-17 | E-GEOD-56813 | biostudies-arrayexpress
Project description:The cardiac conduction system (CCS) comprises a network of highly specialized cells, including sinoatrial node, atrioventricular node, His bundle, bundle branches and Purkinje fibers. To uncover the transcriptomic landscape across the postnatal CCS and comparative profiling of different CCS components, we conducted scRNA-seq in purified postnatal CCS cells and spatial transcriptomic analysis in the postnatal CCS reporter mouse heart.
2024-06-25 | GSE231547 | GEO
Project description:Transcriptomic profiling of the postnatal mouse cardiac conduction system
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