Project description:The mRNA and protein levels of FAM60A were significantly upregulated in PTC cancer tissues compared with paired normal tissue. Knockdown of FAM60A1 in TPC-1 and K1 significantly supressed cell growth, colony formation ability, tumor invasion, and phase transition of the cell cycle. We used microarrays to investigate the gene expression alterations of PTC cells after FAM60A knockdown by shRNA.
Project description:The mRNA and protein levels of SHCBP1 were significantly upregulated in PTC cancer tissues compared with paired normal tissue. Knockdown of SHCBP11 in TPC-1 and K1 significantly supressed cell growth, colony formation ability, tumor invasion, and phase transition of the cell cycle. We used microarrays to investigate the gene expression alterations of PTC cells after SHCBP1 knockdown by shRNA.
Project description:Achaete scute-like 2 (Ascl2), a basic helix-loop-helix (bHLH) transcription factor, controls the fate of intestinal stem cells. However, the role of Ascl2 in colon cancer progenitor cells remains unknown. The cell lines HT-29 (47.5-95% of CD133+ population) and LS174T (0.45% of CD133+ population) were chosen for functional evaluation of Ascl2 in colon cancer progenitor cells after gene knockdown by RNA interference. The microRNA (miRNA) microarrays identified 26 two-fold up-regulated miRNAs and 58 two-fold down-regulated miRNAs in shRNA-Ascl2/HT-29 cells and 178 two-fold up-regulated miRNAs and 172 two-fold down-regulated miRNAs in shRNA-Ascl2/LS174T cells. Two-condition experiment: shRNA-Ascl2/HT-29 cells vs. shRNA-Ctr/HT-29 cells, and shRNA-Ascl2/LS174T cells vs. shRNA-Ctr/LS174T cells. Biological replicates: 1 HT-29 cells stably transfected with shRNA-Ascl2/EGFP, 1 LS174T cells stably transfected with shRNA-Ascl2/EGFP, 1 HT-29 cells stably transfected with shRNA-Control/EGFP, and 1 LS174T cells stably transfected with shRNA-Control/EGFP, independently grown and harvested. One replicate per array.
Project description:An RNA transcriptome sequencing analysis was performed in MKN45 cells that were transfected with tcons_00001221 shRNA or control shRNA.
Project description:RNA transcriptome sequencing analysis was performed in SGC-7901 cells that were transfected with ENST00000431060 shRNA or control shRNA
Project description:a RNA transcriptome sequencing analysis was performed in primary human keratinocytes that were transfected with shRNA-NR037661 or control shRNA