Project description:Two tebuconazole adaptors, TJ1503 and TJ1669, were unstable. On YPD-agar plates, they gave rise to small (S) and large (L) colonies. We sequenced both types of colonies. TJ1503S and TJ1669S were the S colonies. TJ1503L and TJ1669L were the large colonies.
Project description:In Candida albicans, one strain (TJ1031) has segmental monosomy of chromosome R from rDNA to the right tolemere. This strain was unstable, yielding small and large colonies on YPD plate. Randomly 5 large colonies were sequenced.
Project description:In Candida albicans, aneuploids are unstable. When grown on YPD-agar plates, aneuploids exhibit colony size variations. In this study, 6 aneuploid strains were spread on YPD-agar plates. The large colonies from each strain were sequenced. The small colonies were sequenced in another project.
Project description:We report the development and application of isogenic colony sequencing to profile heterogeneity among yeast colonies. We profiled transcriptomes of the opaque-white switching in C. albicans colonies and an ARO4 mutagenesis library in S. cerevisiae colonies.
Project description:Candida albicans lab strain SC5314 was exposued to sub-inhibitory amount (0.5 ug/ml) fluconazole for 24h. Two tolerant adaptors (FY1284 and FY1285) were obtained. The adaptors were unstable on YPD plate, yielding small (S) and large (L) colonies. The small (FY1284-S and FY1285-S) and the large (FY1284-L and FY1285-L) colonies were sequenced
Project description:Candida albicans lab strain SC5314 was grown in YPD broth supplemented with sub-MIC concentration (5 ng/ml) of aureobasidin A (AbA) for 24h. The culture was then washed and diluted with distilled water. Approximately 200 cells were spread on YPD agar plate. Randomly 40 colonies were tested with spot assay for tolerance to AbA. 21 colonies were tolerant and were sequenced.
Project description:Human multipotent stromal cells readily form single-cell-derived colonies when plated at clonal densities. However, the colonies are heterogeneous because cells from a colony form new colonies that vary in size and differentiation potential when replated at clonal densities. The experiments here tested the hypothesis that cells in the inner regions of colonies are partially differentiated, but the differentiation is reversible. Cells were separately isolated from the dense inner (IN) regions and less-dense outer regions (OUT) of single-cell-derived colonies. Cells were then compared by assays of their transcriptomes and proteins, and for clonogenicity and differentiation. IN cells expressed fewer cell-cycle genes and higher levels of genes for extracellular matrix than the OUT cells. When transferred to differentiation medium, differentiation of the colonies occurred primarily in the IN regions. However, the IN cells were indistinguishable from OUT cells when replated at clonal densities and assayed for rates of propagation and clonogenicity. Also, colonies formed by IN cells were similar to colonies formed by OUT cells because they had distinct IN and OUT regions. Cultures of IN and OUT cells remained indistinguishable through multiple passages (30-75 population doublings), and both cells formed colonies that were looser and less dense as they were expanded. The results demonstrated that cells in the IN region of single-cell-derived colonies are partially differentiated, but the differentiation can be reversed by replating the cells at clonal densities.