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NRibo-Seq timecourse in RAW264.7 cells

ABSTRACT: nRibo-Seq timecourse in RAW264.7 cells

PROVIDER: PRJNA649002 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12333547.fastq.gz	Fastqsanger.gz
	SRR12333548.fastq.gz	Fastqsanger.gz
	SRR12333549.fastq.gz	Fastqsanger.gz
	SRR12333550.fastq.gz	Fastqsanger.gz
	SRR12333551.fastq.gz	Fastqsanger.gz

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Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the gene expression of E4BP4 knockout (KO) RAW264.7 cells. Methods: We generated E4BP4-KO RAW264.7 Cell Line using CRISPR-CAS9 system. Control plasmid was encoding the Cas9 nuclease without gRNA sequence. Total RNA of E4BP4-KO and Control (CTRL) RAW264.7 cells was extracted. RAW264.7 cells RNA profiles were generated by deep sequencing for two groups (E4BP4-KO versus CTRL RAW264.7 cells) with three samples each. Results: There were significant differences between E4BP4-KO and CTRL RAW264.7 cells. Conclusions: Deletion of E4BP4 in RAW264.7 cells induced various changes at the transcription level.

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