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Expression profiles of E4BP4 knockout RAW264.7 cells [RAW_KOvsWT_RNA-seq]

ABSTRACT: Expression profiles of E4BP4 knockout RAW264.7 cells [RAW_KOvsWT_RNA-seq]

PROVIDER: PRJNA759126 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR15676420.fastq.gz	Fastqsanger.gz
	SRR15676421.fastq.gz	Fastqsanger.gz
	SRR15676422.fastq.gz	Fastqsanger.gz
	SRR15676423.fastq.gz	Fastqsanger.gz
	SRR15676424.fastq.gz	Fastqsanger.gz

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Expression profiles of E4BP4 knockout RAW264.7 cells [RAW_KOvsWT_RNA-seq]

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the gene expression of E4BP4 knockout (KO) RAW264.7 cells. Methods: We generated E4BP4-KO RAW264.7 Cell Line using CRISPR-CAS9 system. Control plasmid was encoding the Cas9 nuclease without gRNA sequence. Total RNA of E4BP4-KO and Control (CTRL) RAW264.7 cells was extracted. RAW264.7 cells RNA profiles were generated by deep sequencing for two groups (E4BP4-KO versus CTRL RAW264.7 cells) with three samples each. Results: There were significant differences between E4BP4-KO and CTRL RAW264.7 cells. Conclusions: Deletion of E4BP4 in RAW264.7 cells induced various changes at the transcription level.

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Project description:E4BP4 was overexpressed in RAW264.7 cells using lentivirus and RNAseq was performed.

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Project description:ATACseq was performed by overexpressing E4BP4 in RAW264.7 cells using lentivirus.

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ChIP_seq for E4BP4 in RAW264.7 cells [RAW_ChIP-seq]

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Assay for Transposase-Accessible Chromatin in E4BP4 overexpressed RAW264.7 cells

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Hepatocyte E4BP4 promotes liver fibrosis via enhancing hepatic stellate cells activation

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