Project description:Fecal Exosomes from RCD and HFD (isolated from 12 months of their respective diets and purified by sucrose gradient) were orally gavaged to B6 mice for 14 days while they fed HFD. These exosomes were characterized for CD63+A33 duol positivity. After 14 days, liver tissue were harvested. RNA was isolated and sent to invitorgen for Affymetrix array. The purpose of the experiment was to see which set of genes go up or down after these exosomes treatment and their link to metabolic syndrome.
Project description:MBELN isolated mulberry bark purified by sucrose gradient were orally gavaged to B6 mice for 7 days once in a day at aconcentration of 10 x 10E+09 particle/100 µl. After 7 days, colon tissue were harvested. RNA was isolated and sent to invitorgen for Affymetrix array. The purpose of the experiment was to see which set of genes go up or down after MBELN treatment and their link to gut homeostasis.
Project description:MBELN isolated mulberry bark purified by sucrose gradient were orally gavaged to B6 mice for 7 days once in a day at aconcentration of 10 x 10E+09 particle/100 µl. After 7 days, colon specific crypt cells were isolated. RNA was isolated and sent to invitorgen for Affymetrix array. The purpose of the experiment was to see which set of genes go up or down after MBELN treatment and their link to gut homeostasis.
Project description:This study aimed to analyze changes in gut microbiota composition in mice after transplantation of fecal microbiota (FMT, N = 6) from the feces of NSCLC patients by analyzing fecal content using 16S rRNA sequencing, 10 days after transplantation. Specific-pathogen-free (SPF) mice were used for each experiments (N=4) as controls.
Project description:Wildtype B6, Rag1-/- B6 and Rag1-/- B6 mice harboring the 225.4 IgA producing hybridoma were colonized for 10 days with Bacteroides thetaiotaomicron Experiment Overall Design: Distal small intestine segments were snap frozen immediately after the mice were sacrificed, and small intestine divided into 16 equal segments, segments 10, 11, 12 and 14 were pooled and RNA extracted.