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RNAseq for Identification of Doxycycline transcriptomic impact in N2 & CB4856 C. elegans worms

ABSTRACT: RNAseq for Identification of Doxycycline transcriptomic impact in N2 & CB4856 C. elegans worms

PROVIDER: PRJNA661536 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR12590304.fastq.gz	Fastqsanger.gz
	SRR12590305.fastq.gz	Fastqsanger.gz
	SRR12590306.fastq.gz	Fastqsanger.gz
	SRR12590307.fastq.gz	Fastqsanger.gz
	SRR12590308.fastq.gz	Fastqsanger.gz

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Project description:We analyzed the consequences of recombination in gene expression. For this, we measured expression patterns in two C. elegans wild types, N2 and CB4856 (CB). We compare these profiles with gene expression values from a Recombinant Inbred Population (RIL) derived from the same wild types. Expression values of the RILs (GSE17071) were obtained from Viñuela et al. (Genome Research, 2010). N2 and CB strain nematodes were cultured in identical conditions. Likewise, mRNA was extracted at three different ages (juveniles, reproductive and old worms) to investigate the transcriptional consequences of recombination in aging worms. Then, we explored gene expression heritability and transgression as genetic parameters for the analysis of gene expression divergence in natural isolates. Moreover, we investigated the progression of those parameters with age.

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