Foxc1 establishes enhancer accessibility for craniofacial cartilage differentiation
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA661770 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR12601600_1.fastq.gz | Fastqsanger.gz | |||
| SRR12601600_2.fastq.gz | Fastqsanger.gz | |||
| SRR12601601_1.fastq.gz | Fastqsanger.gz | |||
| SRR12601601_2.fastq.gz | Fastqsanger.gz | |||
| SRR12601602_1.fastq.gz | Fastqsanger.gz |
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Project description:The specification of cartilage requires Sox9, a transcription factor with broad roles for organogenesis outside the skeletal system. How Sox9 and other factors gain access to cartilage-specific cis-regulatory regions during skeletal development was unknown. By analyzing chromatin accessibility during the differentiation of neural crest cells into chondrocytes of the zebrafish head, we find that cartilage-associated chromatin accessibility is dynamically established. Cartilage-associated regions that become accessible after neural crest migration are co-enriched for Sox9 and Fox transcription factor binding motifs. In zebrafish lacking Foxc1 paralogs, we find a global decrease in chromatin accessibility in chondrocytes, consistent with a later loss of dorsal facial cartilages. Zebrafish transgenesis assays confirm that many of these Foxc1-dependent elements function as enhancers with region- and stage-specific activity in facial cartilages. These results show that Foxc1 promotes chondrogenesis in the face by establishing chromatin accessibility at a number of cartilage-associated gene enhancers.
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