Project description:Poor reproducibility within and across studies arising from lack of knowledge regarding the performance of extracellular RNA (exRNA) isolation methods has hindered progress in the exRNA field. A systematic comparison of 10 exRNA isolation methods across 5 biofluids revealed marked differences in the complexity and reproducibility of the resulting small RNA-seq profiles. The relative efficiency with which each method accessed different exRNA carrier subclasses was determined by estimating the proportions of extracellular vesicle (EV)-, ribonucleoprotein (RNP)-, and high-density lipoprotein (HDL)-specific miRNA signatures in each profile. An interactive web-based application (miRDaR) was developed to help investigators select the optimal exRNA isolation method for their studies. miRDar provides comparative statistics for all expressed miRNAs or a selected subset of miRNAs in the desired biofluid for each exRNA isolation method and returns a ranked list of exRNA isolation methods prioritized by complexity, expression level, and reproducibility. These results will improve reproducibility and stimulate further progress in exRNA biomarker development.

| S-EPMC6557167 | biostudies-literature

ExRNA Atlas Analysis Reveals Distinct Extracellular RNA Cargo Types Present Across Human Biofluids

Project description:ExRNA Atlas Analysis Reveals Distinct Extracellular RNA Cargo Types Present Across Human Biofluids

| PRJNA510084 | ENA

Assessment of RNA Isolation Kit Biases Toward Preferential Detection of exRNA Cargo Types

Project description:An integrative analysis of human biofluid data in the exRNA Atlas revealed the existence of distinct extracellular RNA cargo types. To determine whether different RNA isolation kits biased detection of certain exRNA cargo types, an integrative analysis was performed using pooled plasma and serum samples, where 10 different RNA isolation kits were applied.

2018-12-15 | GSE123865 | GEO

microRNA expression in human traumatic brain injury biofluids

Project description:Purpose: The goal of this study is to investigate the expression of microRNAs in TBI biofluids compared to uninjured controls Methods: miRNA-Seq analysis of microRNA expression TBI and uninjured control biofluids

2020-03-09 | GSE131695 | GEO

ExRNA in Biofluids as Biomarkers for Brain Tumors.

Project description:Patients with high-grade gliomas and glioblastomas (GBMs) have poor survival despite optimal surgical and drug therapy. Minimally invasive diagnostic biomarkers would enable early diagnosis and tumor-specific treatments for 'personalized targeted' therapy, and would create the basis for response tracking in patients with GBM. Extracellular vesicles (EVs) isolated from cerebrospinal fluid and blood contain glioma-specific molecules, including tumor-derived EV RNAs that are detectable in small copy numbers in these biofluids. EV RNA mutations or expression changes are also detectable, the analysis of which gives rise to 'liquid biopsy' tumor profiling.

| S-EPMC5451268 | biostudies-literature

Large differences in small RNA composition between human biofluids - 10 biofluids

Project description:One goal of Phase 1 of our project is to produce reference profiles of miRNAs and other small RNAs in a large and diverse set of biofluids using a systemic approach. This study contains samples associated with 10 different biofluids, and all data (sequencing data, full alignments, etc.) is openly available.

2018-03-27 | GSE112343 | GEO