Project description:One goal of Phase 1 of our project is to produce reference profiles of miRNAs and other small RNAs in a large and diverse set of biofluids using a systemic approach. This study contains samples associated with 10 different biofluids, and all data (sequencing data, full alignments, etc.) is openly available.

2018-03-27 | GSE112343 | GEO

Small RNA Sequencing across Diverse Biofluids Identifies Optimal Methods for exRNA Isolation.

Project description:Poor reproducibility within and across studies arising from lack of knowledge regarding the performance of extracellular RNA (exRNA) isolation methods has hindered progress in the exRNA field. A systematic comparison of 10 exRNA isolation methods across 5 biofluids revealed marked differences in the complexity and reproducibility of the resulting small RNA-seq profiles. The relative efficiency with which each method accessed different exRNA carrier subclasses was determined by estimating the proportions of extracellular vesicle (EV)-, ribonucleoprotein (RNP)-, and high-density lipoprotein (HDL)-specific miRNA signatures in each profile. An interactive web-based application (miRDaR) was developed to help investigators select the optimal exRNA isolation method for their studies. miRDar provides comparative statistics for all expressed miRNAs or a selected subset of miRNAs in the desired biofluid for each exRNA isolation method and returns a ranked list of exRNA isolation methods prioritized by complexity, expression level, and reproducibility. These results will improve reproducibility and stimulate further progress in exRNA biomarker development.

| S-EPMC6557167 | biostudies-literature

Colorectal cancer cell supermeres isolation methods

Project description:Colorectal cancer cell supermeres isolation methods

| PRJNA1136963 | ENA

Total RNA sequencing in human biofluids and extracellular vesicles using SMARTer technology

Project description:Transcriptome profiling has emerged as a powerful tool to investigate the biomarker potential of human biofluids. However, despite an enormous interest in extracellular nucleic acids, total RNA sequencing methods to quantify RNA content outside cells are rare. Here, we evaluate the performance of the SMARTer Stranded Total RNA-Seq method in human platelet-rich plasma, platelet-poor plasma, urine, conditioned medium, and extracellular vesicles from these biofluids.

2019-09-01 | GSE131689 | GEO

microRNA expression in human traumatic brain injury biofluids

Project description:Purpose: The goal of this study is to investigate the expression of microRNAs in TBI biofluids compared to uninjured controls Methods: miRNA-Seq analysis of microRNA expression TBI and uninjured control biofluids

2020-03-09 | GSE131695 | GEO

Large differences in small RNA composition between human biofluids - 10 biofluids

Project description:Large differences in small RNA composition between human biofluids - 10 biofluids

| PRJNA445720 | ENA

Comparison of EVs isolation methods for miRNAs sequencing

Project description:Comparison of EVs isolation methods for miRNAs sequencing

| PRJNA928492 | ENA