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Escherichia coli O104:H4 str. LB226692

ABSTRACT: Escherichia coli O104:H4 str. LB226692 Genome sequencing and assembly

PROVIDER: PRJNA67613 | ENA |

REPOSITORIES: ENA

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			Action	DRS
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	SRR254209.fastq.gz	Fastqsanger.gz

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Project description:We performed a high-throughput mapping of the 5’ end transcriptome of the pAA plasmid of the clinical Escherichia coli O104:H4 (E. coli O104:H4) isolate LB226692. We employed differential RNA-sequencing (dRNA-seq), a terminator exonuclease (TEX)-based RNA-seq approach allowing for the discrimination of primary and processed transcripts. This method has proven to be a powerful tool for the mapping of transcription start sites (TSS) and detection of non-coding RNAs (ncRNAs) in bacteria. We catalogued pAA-associated TSS and processing sites on a plasmid-wide scale and performed a detailed analysis of the primary transcriptome focusing on pAA virulence gene expression.

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