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Next Generation Sequencing Facilitates Quantitative Analysis of oral epithelial cells from SPF 8 weeks and 1 week old Wild Type mice

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of oral epithelial cells from SPF 8 weeks and 1 week old Wild Type mice

PROVIDER: PRJNA680243 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13109089.fastq.gz	Fastqsanger.gz
	SRR13109090.fastq.gz	Fastqsanger.gz
	SRR13109091.fastq.gz	Fastqsanger.gz
	SRR13109092.fastq.gz	Fastqsanger.gz
	SRR13109093.fastq.gz	Fastqsanger.gz

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Project description:The role of microbe in promoting the initiation of gastric cancer (GC), the third most lethal cancer worldwide, are ill-defined. Here, we found that tumor size and weight in gp130F/F mouse stomach at condition were significantly reduced compared to those of at SPF condition. To investigate the underlying mechanism and how host genes were regulated in the presence/absence of microbe, arrays were performed in stomach tissue from gp130F/F and WT at 4 week old at SPF and GF conditions. The onset of gastric inflammation-associated adenomatous hyperplasia in gp130F/F mice occurs at ~6 weeks of age, with established intestinal-type tumors (3 months of age) progressively growing until a maximum size is reached at 6 months of age, with some evidence of carcinoma in situ. Since this phenotype histologically mimics early stage human GC, we therefore utilized gp130F/F mice as a model to identify miRNAs involved in the initiating molecular events leading to early stage GC. For this purpose, we performed miRNA microarrays on mouse gastric antrum tissue from gp130F/F and sex-matched littermate wild-type (gp130+/+) control mice aged 4 weeks; this age was chosen since antrum tissue from 4 week old (wo) gp130F/F mice is devoid of any histological signs of inflammation or hyperplasia, and is thus comparable to wild-type gastric antrum tissue.

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