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Dimorphic H3K27 methylation guides germline or vegetative cell fate dichotomy in male gametophytes [CUT&Tag]

ABSTRACT: Dimorphic H3K27 methylation guides germline or vegetative cell fate dichotomy in male gametophytes [CUT&Tag]

PROVIDER: PRJNA682443 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13195578_1.fastq.gz	Fastqsanger.gz
	SRR13195578_2.fastq.gz	Fastqsanger.gz
	SRR13195579_1.fastq.gz	Fastqsanger.gz
	SRR13195579_2.fastq.gz	Fastqsanger.gz
	SRR13195580_1.fastq.gz	Fastqsanger.gz

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Dimorphic H3K27 methylation guides germline or vegetative cell fate dichotomy in male gametophytes

Project description:Dimorphic H3K27 methylation guides germline or vegetative cell fate dichotomy in male gametophytes

| PRJNA682442 | ENA

Dimorphic H3K27 methylation guides germline or vegetative cell fate dichotomy in male gametophytes [ATAC-seq]

Project description:Dimorphic H3K27 methylation guides germline or vegetative cell fate dichotomy in male gametophytes [ATAC-seq]

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Dimorphic H3K27 methylation guides germline or vegetative cell fate dichotomy in male gametophytes [RNA-seq]

Project description:Dimorphic H3K27 methylation guides germline or vegetative cell fate dichotomy in male gametophytes [RNA-seq]

| PRJNA682445 | ENA

Dimorphic H3K27 methylation guides germline or vegetative cell fate dichotomy in male gametophytes [RNA-seq]

Project description:During angiosperm male gametogenesis, microspores divide to produce a vegetative cell (VC) and a male germline (MG), each with a distinct cell fate. How the MG cell/VC fate is determined remains largely unknown. Here, we report that the VC-targeted H3K27me3 erasure resulted in VC fate transition towards a gamete destination. Multi-omics and cytologic analysis reveal that H3K27me3 is essential for VC fate commitment and contributes to suppress the MG cell fate initiation in VC, whereas MG cells require H3K27me3 reprograming for the gamete cell fate. This work suggests that the MG cell/VC fate is epigenetically regulated. The dimorphic H3K27 methylation acts as a core switch to determine their distinct cell fates and ensure the functional specification of both VC and MG for pollen fertility. This work also provides direct evidences for the proposal that VC maintains the default developmental program of microspore, whereas MG requires reprogramming.

2022-08-03 | GSE162639 | GEO

Dimorphic H3K27 methylation guides germline or vegetative cell fate dichotomy in male gametophytes [ATAC-seq]

2022-08-03 | GSE162638 | GEO

Dimorphic H3K27 methylation guides germline or vegetative cell fate dichotomy in male gametophytes [CUT&Tag]

2022-08-03 | GSE162637 | GEO

Dimorphic H3K27 methylation guides germline or vegetative cell fate dichotomy in male gametophytes

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-08-03 | GSE162640 | GEO

Temperature dependent sex-biased gene expression in the gametophytes of the kelp Saccharina latissima

Project description:We performed a laboratory experiment with vegetative gametophytes of the kelp Saccharina latissima and exposed the gametophytes to three temperatures (4°C, 12°C and 20°C) by sex (female, male) for 14 days.

2019-09-25 | E-MTAB-8267 | biostudies-arrayexpress

Comparison of C. cinerea vegetative dikaryotic to vegetative monokaryotic mycelia

Project description:Coprinopsis cinerea vegetative dikaryotic mycelia compared to vegetative monokaryotic mycelia

2010-02-01 | GSE18540 | GEO

Single-nucleus RNA-seq reveals that MBD5, MBD6, and SILENZIO maintain silencing in the vegetative cell of developing pollen

Project description:Silencing of transposable elements (TEs) drove the evolution of numerous redundant mechanisms of transcriptional regulation. Arabidopsis MBD5, MBD6, and SILENZIO act as TE repressors downstream of DNA methylation. Here we show via single-nucleus RNA-seq of developing male gametophytes that these repressors are critical for TE silencing in the pollen vegetative cell, which undergoes epigenetic reprogramming causing chromatin decompaction to support fertilization by sperm cells. Instead, other silencing mutants (met1, ddm1, mom1, morc) show loss of silencing in all pollen nucleus types and somatic cells. We found that TEs repressed by MBD5/6 gain accessibility in wild-type vegetative nuclei despite remaining silent, suggesting that loss of DNA compaction makes them sensitive to loss of MBD5/6. Consistently, crossing mbd5/6 to histone 1 mutants, which have decondensed chromatin in leaves, reveals derepression of MBD5/6-dependent TEs in leaves. MBD5/6 and SILENZIO thus act as a silencing system especially important when chromatin compaction is compromised.

2022-10-27 | GSE202422 | GEO

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