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RNA sequencing reveals that methionine supply during late-gestation alters neonatal Holstein heifer calf liver transcriptome profiles

ABSTRACT: RNA sequencing reveals that methionine supply during late-gestation alters neonatal Holstein heifer calf liver transcriptome profiles

PROVIDER: PRJNA686992 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13282309.fastq.gz	Fastqsanger.gz
	SRR13282310.fastq.gz	Fastqsanger.gz
	SRR13282311.fastq.gz	Fastqsanger.gz
	SRR13282312.fastq.gz	Fastqsanger.gz
	SRR13282313.fastq.gz	Fastqsanger.gz

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RNA sequencing reveals that methionine supply during late-gestation alters neonatal Holstein heifer calf liver transcriptome profiles

Project description:Maternal methionine supply during late pregnancy can affect offspring growth and development via nutritional programming. In the present study, we investigated if increasing the supply of methionine during late-pregnancy in Holstein cows affects transcriptome profiles in the offspring.

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Effect of early-life plane of nutrition on the hepatic transcriptional profile in heifer calves

Project description:The objective of this study was to examine changes in hepatic gene expression of heifer calves offered varying planes of nutrition during early life. At approximately 3 weeks of age (19 ± 5 days of age) Angus X Holstein-Friesian heifer calves were offered a high (HP, n=15) or moderate plane of nutrition (MP, n=15) to achieve target growth rates of 1.2 kg/d and 0.5 kg/d, respectively. After 18 weeks of differential feeding all calves were euthanized and the liver of each calf recovered. RNA was extracted and subsequently subjected to RNAseq analysis. A total of 591 genes were identified as significantly differentially expressed between HP and MP calves. Differentially expressed genes were involved in biological pathways and processes including those related to metabolism (amino acid, vitamin and mineral metabolism) as well as those involved in organ growth and development.

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Effect of early-life plane of nutrition on the transcriptional profile of the hypothalamic arcuate nucleus region in heifer calves

Project description:The aim of this study was to examine alterations in the transcriptional profile of the arcuate nucleus region of the hypothalamus between heifer calves offered varying planes of nutrition during early life. Angus X Holstein-Friesian heifer calves (19 ± 5 days of age, BW: 51.2 ± 7.8 kg, mean ± SD) were offered a high (HP, n=14) or moderate plane of nutrition (MP, n=15) from 3 to 21 weeks of age to achieve a target growth rate of 1.2 kg/d and 0.5 kg/d, respectively. At 21 weeks of age, all calves were euthanized and the arcuate nucleus region of the hypothalamus of each calf recovered. RNA was extracted transcriptional profiles examined using RNAseq. Compared with calves on the moderate plane of nutrition, a High plane of nutrition altered the expression of 80 genes within the arucate nucleus. Functional analysis of differentially expressed genes revealed functions including organismal development, behaviour, endocrine system development and function, lipid metabolism, molecular transport, small molecule biochemistry, cellular development, cellular growth and proliferation, and nervous system development and function to be affected by early life plane of nutrition in the arucate nucleus of heifer calves.

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Transcriptomic analysis of sheep hypothalamus on sex-dependent differences in body weight of progeny born to dams supplemented with omega-3 fatty acids or methionine during late-gestation

Project description:Feeding dams during gestation affects the development of the offspring for their entire life. The objective of the current experiment was to evaluate the changes of the transcriptome in the hypothalamus of the offspring lambs born from dams supplemented with: i) a control diet (without lipids or methionine supplementation), ii) an omega 3 fatty acid supplementation, or iii) a methionine supplementation. The supplementation took place in the last third of gestation and the hypothalamus of male and female offspring was collected after being on a fattening diet for 54 days. Hypothalamus samples were used to extract RNA and analyzed using RNA sequencing. There was an interaction due to sex and methionine supplementation. The pathways that were modified were chromatin structure, developmental processes, and organ morphology. The modification observed on these pathways could explain the sex-by-treatment interaction differences previously observed in growth. There was no sex by omega-3 fatty acid interaction on the hypothalamus transcriptome. Therefore, the sexual dimorphism observed by methionine supplementation may be regulated by the hypothalamus.

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Effect of early-life plane of nutrition on the transcriptional profile of visceral adipose tissue in heifer calves

Project description:The aim of this study was to examine changes in the transcriptional profile of visceral adipose tissue in heifer calves offered varying planes of nutrition during the early life period. From approximately 3 weeks of age (19 ± 5 days of age) Angus X Holstein-Friesian heifer calves were offered either a high (HP, n=15) or moderate (MP, n=15) plane of nutrition to achieve target growth rates of 1.2 kg/d and 0.5 kg/d, respectively. After 18 weeks of differential feeding all calves were euthanized and visceral adipose tissue samples harvested from each calf. RNA was extracted and subsequently subjected to RNAseq analysis. A total of 1052 genes were identified as significantly differentially expressed between calves on the High plane of nutrition and those on the Moderate plane of nutrition. Biological pathway analysis revealed an enrichement from these genes for processes and pathways including Sirtuin signalling, oxidative phosphorylation and adipogenesis.

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Effect of early-life plane of nutrition on the transcriptional profile of the anterior pituitary gland in heifer calves

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Effect of early-life plane of nutrition on the transcriptional profile of the hypothalamus in heifer calves

Project description:The objective of this study was to examine changes in hypothalamic gene expression of heifer calves offered varying planes of nutrition during early life. Angus X Holstein-Friesian heifer calves (19 ± 5 days of age, BW: 51.2 ± 7.8 kg, mean ± SD) were offered a high (HP, n=14) or moderate plane of nutrition (MP, n=15) from 3 to 21 weeks of age to achieve a target growth rate of 1.2 kg/d and 0.5 kg/d, respectively. At 21 weeks of age, all calves were euthanized and the hypothalamus of each calf recovered. RNA was extracted and hypothalamic gene expression examined using RNAseq. Compared with calves on the moderate plane of nutrition, a HP of nutrition altered the expression of 39 transcripts in the hypothalamus. Biological pathway analysis revealed these differentially expressed genes to be involved in pathways including acute phase response signalling, STAT3 pathway, and tight junction signalling. Additionally, biological functions affected by early life plane of nutrition included cell-to-cell signalling and interaction, molecular transport, small molecule biochemistry, behaviour, nervous system development and function, nucleic acid metabolism, and cellular function and maintenance.

2021-07-14 | GSE153495 | GEO

Maternal methionine supplementation alters the epigenome of the offspring [RNA-Seq]

Project description:Maternal nutrition can induce modifications in the epigenome of the fetus, resulting in gene expression alterations and impacting future performance. Epigenetic changes are related to nutrition via the one carbon cycle, which involves aminoacid methionine. We hypothesized that an increased dietary methionine in beef cows during early gestation will impact the whole-genome DNA methylation and transcriptome of the offspring.

2018-07-13 | GSE116974 | GEO