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ABSTRACT: Raw reads of Loropetalum chinense var. rubrum chloroplast complete genome

PROVIDER: PRJNA689662 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13356418_1.fastq.gz	Fastqsanger.gz
	SRR13356418_2.fastq.gz	Fastqsanger.gz

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Project description:BACKGROUND: Loropetalum chinense var. rubrum (L. chinense var. rubrum) is a precious, native, coloured-leaf ornamental plant in the Hunan Province. We found a L. chinense var. rubrum tree with 3 different leaf colours: GL (green leaf), ML (mosaic leaf) and PL (purple leaf). The mechanism of leaf coloration in this plant is still unclear. The aim of this study was to identify the metabolites and genes involved in determining the colour composition of L. chinense var. rubrum leaves, using phenotypic/anatomic observations, pigment content detection, and comparative metabolomics and transcriptomics.RESULTS: We observed that the mesophyll cells in PL were purple, those in GL were green and those in ML were a mix of purple-green. The contents of chlorophyll a, b, carotenoids and total chlorophyll in PL and ML were significantly lower than those in GL. While the anthocyanin content in PL and ML was significantly higher than that in GL. The metabolomics results showed that the differences in content of cyanidin 3-O-glucoside, delphinidin 3-O-glucoside, cyanidin 3,5-O-diglucoside, pelargonin and petunidin 3,5-diglucoside in ML, GL and PL were significant. Considering that the trend of anthocyanin content change was consistent with the leaf colour difference, we speculated that these compounds might influence the colour of L. chinense var. rubrum leaves. Finally, using transcriptomics, we identified 9 differentially expressed structural genes (one ANR (ANR1217); four CYP75A (CYP75A1815, CYP75A2846, CYP75A2909 and CYP75A1716); four UFGTs (UFGT1876, UFGT1649, UFGT1839 and UFGT3273) and nine transcription factors (two MYBs (MYB1057 and MYB1211), one MADS-box (MADS1235), two AP2-likes (AP2-like1779 and AP2-like2234), one bZIP (bZIP3720), two WD40s (WD2173 and WD1867) and one bHLH (bHLH1631) that might be related to flavonoid biosynthesis that impacted the appearance of colour in L. chinense var. rubrum leaves.CONCLUSIONS: This study revealed potential molecular mechanisms associated with leaf coloration in L. chinense var. rubrum by analysing differential metabolites and genes related to the anthocyanin biosynthesis pathway. It also provides a reference for research on leaf colour variation in other ornamental plants.

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