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RNA-seq and physiology analysis reveal new insights into gill response to acute hypoxia in Hybrid Yellow Catfish (Tachysurus fulvidraco ♀ × Pseudobagrus vachellii ♂)

ABSTRACT: RNA-seq and physiology analysis reveal new insights into gill response to acute hypoxia in Hybrid Yellow Catfish (Tachysurus fulvidraco ♀ × Pseudobagrus vachellii ♂)

PROVIDER: PRJNA699150 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13617752_1.fastq.gz	Fastqsanger.gz
	SRR13617752_2.fastq.gz	Fastqsanger.gz
	SRR13617753_1.fastq.gz	Fastqsanger.gz
	SRR13617753_2.fastq.gz	Fastqsanger.gz
	SRR13617754_1.fastq.gz	Fastqsanger.gz

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The skin transcriptome analysis of yellow catfish (Tachysurus fulvidraco♀ × P. vachellii♂) under transport stress

Project description:The skin transcriptome analysis of yellow catfish (Tachysurus fulvidraco♀ × P. vachellii♂) under transport stress

| PRJNA743861 | ENA

The gills transcriptome analysis of yellow catfish (Tachysurus fulvidraco♀ × P. vachellii♂) under transport stress

Project description:The gills transcriptome analysis of yellow catfish (Tachysurus fulvidraco♀ × P. vachellii♂) under transport stress

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The intestine transcriptome analysis of hybrid yellow catfish (Tachysurus fulvidraco♀ × P. vachellii♂) under transport stress

Project description:The intestine transcriptome analysis of hybrid yellow catfish (Tachysurus fulvidraco♀ × P. vachellii♂) under transport stress

| PRJNA849418 | ENA

Changes in Growth Performance, Physiological Parameters, and Lipid Metabolism-related Genes Expression of Hybrid Yellow Catfish (Tachysurus fulvidraco ♀ × Pseudobagrus vachellii ♂) following different dietary Acanthopanax Senticosus levels

Project description:Changes in Growth Performance, Physiological Parameters, and Lipid Metabolism-related Genes Expression of Hybrid Yellow Catfish (Tachysurus fulvidraco ♀ × Pseudobagrus vachellii ♂) following different dietary Acanthopanax Senticosus levels

| PRJNA647836 | ENA

Growth performance, physiological parameters, and transcript levels of lipid metabolism-related genes in hybrid yellow catfish (Tachysurus fulvidraco ♀ × Pseudobagrus vachellii ♂) fed diets containing Siberian ginseng.

Project description:In high-density aquaculture, fish health can suffer because of excessive feeding, which causes fatty liver disease. Siberian ginseng (Acanthopanax senticosus) has been used as a feed additive to promote animal growth, immunity, and lipid metabolism. In this study, we explored the effects of A. senticosus on the physiology of hybrid yellow catfish (Tachysurus fulvidraco ♀ × Pseudobagrus vachellii ♂). A control group and five groups fed diets containing A. senticosus (0.5, 1, 2, 4, and 8 g A. senticosus/kg feed) were established and maintained for 8 weeks. Dietary supplementation with A. senticosus at 4 g/kg promoted growth of the hybrid yellow catfish. Serum total cholesterol (TC) and triacylglycerol (TG) levels at 2 g/kg A. senticosus (TC: 1.31 mmol/L; TG: 1.08 mmol/L) were significantly lower than in the control group (TC: 1.51 mmol/L; TG: 1.41 mmol/L), and 4 g/kg A. senticosus (17.20 μmol/g tissue) reduced the liver TG level compared with the control group (21.36 μmol/g tissue) (P <0.05). Comparative transcriptomic analysis of liver tissue between the control group and the group showing optimum growth (4 g/kg A. senticosus) revealed 820 differentially expressed genes and 44 significantly enriched pathways, especially lipid metabolism pathways such as unsaturated fatty acid and fatty acid metabolism. The transcript levels of five lipid metabolism-related genes were determined by quantitative real-time PCR. The results showed that 2-4 g/kg A. senticosus supplementation reduced the FADS2, ELOVL2, CYP24a, and PLPP3 transcript levels and 4 g/kg A. senticosus increased the DIO2 transcript level (P <0.05), leading to altered synthesis of TG and thyroxine and reduced fat deposition in the liver. Our results show that dietary A. senticosus affects the regulation of fat metabolism and promotes the growth of hybrid yellow catfish. A. senticosus is a healthy feed additive, and the appropriate dietary supplementation rate is 2-4 g/kg.

| S-EPMC7877655 | biostudies-literature

The Analysis of RNA-Sequencing Reveals the Immune Regulatory Roles of Key MiRNAs in Intestines of the Hybrid Yellow Catfish (Tachysurus fulvidraco♀ * P. vachellii♂) under transport stress

Project description:The Analysis of RNA-Sequencing Reveals the Immune Regulatory Roles of Key MiRNAs in Intestines of the Hybrid Yellow Catfish (Tachysurus fulvidraco♀ * P. vachellii♂) under transport stress

| PRJNA899465 | ENA

RNA-seq and physiology analysis reveal new insights into gill response to acute hypoxia in Hybrid Yellow Catfish (Tachysurus fulvidraco ♀ × Pseudobagrus vachellii ♂)

Project description:Hypoxia is an important environmental stressor in aquatic ecosystems, with increasingly impacts on global biodiversity. Yellow catfish is an economically important farmed fish in China, which has increased dramatically. We investigated the response of hybrid yellow catfish to hypoxia under experimental conditions and focused on the analysis of the differential expression patterns of specific genes associated with hypoxia response by RNA-seq and qPCR analysis. A total of 1556 genes were captured significantly differentially expressed, and were categorized into immune response and energy metabolism. Functional enrichment analysis revealed the NLR signaling pathway play pivotal roles in hypoxia tolerance and resistance. Our study provides important insights into the physiological acclimation, immune response and defense activity of hybrid yellow catfish under hypoxia challenge.

2021-02-04 | GSE166055 | GEO

The skin transcriptome analysis of yellow catfish (Tachysurus fulvidraco♀ × P. vachellii♂) under transport stress

Project description:The skin transcriptomes were examined by high-throughput sequencing at control group and 16 h transport group. A total of 1577 genes were differentially expressed, including 892 up-regulated and 685 down-regulated. It is interesting to note that the qPCR analysis further confirmed that the skin's immune response to transport stress was activated via Toll-like receptors (TLRs) and NOD-like receptors (NLRs) in skin: the tlr9, mfn2 and ikbke were significantly up-regulated and nfkbia, map3k7cl were significantly down-regulated. Overall results suggested that transport stress. The overall results suggest that the skin responds to transport stress by regulating mucus secretion and activating the immune signal pathway. This study will help to optimize the transport conditions for cultured hybrid yellow catfish.

2021-07-07 | GSE179444 | GEO

The gills transcriptome analysis of yellow catfish (Tachysurus fulvidraco♀ × P. vachellii♂) under transport stress

Project description:Transport stress is a big threat to most teleost fish during production practice, causing mass loss to the aquaculture industry. Fish gills are mucosa-associated lymphoid tissue that directly contacted with water, which is the good choice to study the mechanism of transport stress. Gills transcriptome results revealed 1551 differentially expressed genes (744 up-regulated and 807 down- regulated) between the control and 16h transportation groups. The top 10 enriched KEGG pathways are all immune-related. After qRT-PCR validation, we found that the toll-like receptors and nod-like receptors signaling pathways mediate the gill’s immune response to transport stress: tlr5, tnfɑ, hsp90ɑ, il-1ß and map2k4 were significantly up-regulated and arrdc2 was significantly down-regulated under transport stress. These results suggested that the transport stress altered innate immunity responses and induced metabolic changes in gills of hybrid yellow catfish. In conclusion, the results provide new perspectives on immune response in yellow catfish against transport stress and provide the possibility of mining resistance genes for future optimize transportation plan.

2021-12-29 | GSE192657 | GEO

The intestine transcriptome analysis of hybrid yellow catfish (Tachysurus fulvidraco♀ × P. vachellii♂) under transport stress

Project description:The transport of live fish is a routine operation in aquaculture. However, the effects of transport stress on intestines gene expression profile is poorly understood. The results from the transcriptome analysis revealed that most differentially expression genes (DEGs) were enriched in immune and metabolic related signaling pathways. Toll like receptors is a potential pathway in immune response induced by transport stress. Changes in genes related to lipid metabolism may be related to the increased metabolic expenditure for the normal body functions in a response to transport stress. In addition, changes in gene expression in the control, transport, and recovery groups were revealed that transport-induced local immune damage is reversible while metabolic disorder has hysteresis. These results provide new perspectives on immune and metabolic response in yellow catfish against transport stress and theoretical support for future optimization of their transportation.

2022-06-18 | GSE206148 | GEO

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