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Agilent SurePrint G3 Mouse GE V2.0 Microarray(8*60K,Design ID:074809)

ABSTRACT: Agilent SurePrint G3 Mouse GE V2.0 Microarray(8*60K,Design ID:074809)

PROVIDER: PRJNA699158 | ENA |

REPOSITORIES: ENA

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Agilent SurePrint G3 Mouse GE V2.0 Microarray(8*60K,Design ID:074809)

Project description:Total RNA was quantified by the NanoDrop ND-2000 (Thermo Scientific)and the RNA integrity was assessed using Agilent Bioanalyzer 2100 (Agilent Technologies). The sample labeling, microarray hybridization and washing were performed based on the manufacturer's standard protocols. Briefly, total RNA were transcribed to double strand cDNA, then synthesized into cRNA and labeled with Cyanine-3-CTP. The labeled cRNAs were hybridized onto the microarray. After washing, the arrays were scanned by the Agilent Scanner G2505C (Agilent Technologies).

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Genome-wide mRNA expression status of non-B non-C hepatocellular carcinoma assessed by SurePrint G3 Human GE 8x60K v2 Microarray (Agilent, USA)

Project description:Genome-wide mRNA expression analysis for clinical samples from patients in whom non-B non-C hepatocellular carcinoma developed. A pair of tissues (N=15 pairs) from non-tumor background and tumor was provided from every patient. A platform for this experiment was SurePrint G3 Human GE 8x60K v2 Microarray (Agilent, USA).

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Gene expression profiles in CT26 syngeneic tumor tissues after EP4 antagonist TP-16(BY001), anti-PD-1 antibody or combination treatment

Project description:To explore the mechanisms resposible for the synergistic effect between EP4 antagonist BY001 and anti-PD-1 antibody, we conducted a microarray analysis on gene expression profiles in CT26 syngenetic tumor tissues. We used Agilent SurePrint G3 Mouse GE V2.0 Array to detail gene expression after indicated treatments and identified the molecular mechanism during this process to facilitate further studies.

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CD109 regulates in vivo tumor invasion in lung adenocarcinoma through TGF-β signaling

Project description:CD109 is a glycosylphosphatidylinositol-anchored glycoprotein highly expressed in several types of human malignant tumors including lung cancers. We investigated the in vivo functions of CD109 protein in malignant lung tumors. CD109+/+ and CD109-/- K-ras[LSL-G12D/+];p53[fl/fl] (KP) mice were sacrificed at 20 to 23 weeks of age and total RNA was extracted from the lung tumors. SurePrint G3 Mouse GE Microarray 8×60K Ver.2.0 were performed.

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Project description:Agilent SurePrint G3 Human V2 GE 8×60K microarray-BEAS-2B cells treatment with NiCl2

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Gene expression analysis of stimulated primary ASM cells [mRNA]

Project description:mRNA expression was determined using the Agilent-G4851A SurePrint G3 Human GE 8x60K Microarray

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Expression Profiles of SUM149 cell treated by ACY1215

Project description:Expression Profiles of SUM149 cell treated by ACY1215 at 0h, 3h, 6h and 12h by Agilent SurePrint G3 Human GE v3 8x60K.

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Expression analysis of MADM based glioma mouse model

Project description:Mosaic Analysis with Double Markers (MADM) based glioma mouse model, which homozygously lacks Tp53 and Nf1, spontaneously developed gliomas at the post-natal 90-120 days. Tp53 and Nf1 are among the most frequently mutated genes in human glioma patients. Investigating the expression changes of genes induced by inactivation of Tp53 and Nf1 can be a clue to clarify the mechanism of gliomagenesis. We examined the expressions of glioma in MADM mouse at post-natal 150 days (n=3) and of normal brain in Tp53 and Nf1 wild type mouse at post-natal 150 days (n=2). We used SurePrint G3 Mouse GE 8×60K array slides (G4858A, Agilent Technologies).

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Profile of gene expression in K562 cells exposed to 2 mM sodium valproate for 12 hours using Agilent SurePrint G3 Human GE 8x60K Microarray.

Project description:To explore the mechanism underlying anti-leukaemia effect of sodium valproate, the growth and survival of the K562 cell line were investigated using the Annexin V/PI dual staining method. Global profiles of gene expression in K562 cells exposed to sodium valproate were assessed using gene expression microarray and validated by qRT-PCR. Gene ontology analysis was performed to establish the function of differentially expressed genes. The differentially expressed genes identified by using gene expression array were further used to query the CMAP database to retrieve a ranked list of compounds that act on the same intracellular targets as sodium valproate. A significant increase in cell apoptosis was observed in valproate exposed K562 cells using flow cytometry. A total of 706 transcripts were identified as being significantly differentially expressed. Eight differentially expressed genes that might be involved in apoptosis were verified by qRT-PCR. The significant enrichment analysis of gene ontology terms for the differentially expressed genes revealed that these genes are involved in many important biological processes such as apoptosis. The connectivity map analysis showed gene expression profile in K562 cells exposed to sodium valproate observed in this study was most similar to that of HDACi and PI3K inhibitor in the connectivity map database, suggesting that sodium valproate might exert anti-leukaemic action by inhibiting HDAC as well as inhibiting PI3K pathway. In conclusion, the data obtained in this study might provide the ground for further studies to elucidate the molecular and therapeutic potential of VPA in leukaemia treatment. Gene expression in K562 cell line were measured after exposure to 2 mM valproate for 12 hours or left untreated as a control using Agilent SurePrint G3 Human GE 8x60K Microarray.

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