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Bulk and single RNA sequencing on the role of Igf2bp2 in myeloid-biased HSC

ABSTRACT: Bulk and single RNA sequencing on the role of Igf2bp2 in myeloid-biased HSC

PROVIDER: PRJNA699541 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13631504.fastq.gz	Fastqsanger.gz
	SRR13631505.fastq.gz	Fastqsanger.gz
	SRR13631506.fastq.gz	Fastqsanger.gz
	SRR13631507.fastq.gz	Fastqsanger.gz
	SRR13631508.fastq.gz	Fastqsanger.gz

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Bulk and single RNA sequencing on the role of Igf2bp2 in myeloid-biased HSC

Project description:Using RNA sequencing analysis on myeloid biased HSC, we compared gene expression profiles between WT and Igf2bp2 knock-out mice at young and old age. 1421 differentially expressed genes were identified in young myeloid biased HSC from Igf2bp2 knock-out mice compared to young WT; however, only 26 differentially expressed genes were identified in old myeloid biased HSC from Igf2bp2 knock-out mice compared to old WT. Compared to young WT myeloid biased HSC, myeloid biased HSC from young Igf2bp2 knock-out exhibits reduced expression of genes involved in metabolism and translation. In addition, single cell RNA sequencing analysis of myeloid biased HSC in young wildtype mice identified nine sub-clusters. HSCs from a sub-cluster with high expression of Igf2bp2 exhibit up-regulated IGF/PI3K/AKT signaling and increased expression of genes involved in HSC quiescence and maintenance. Altogether, the study provides a correlation and mechanism to understand the role of Igf2bp2 in HSC function and aging.

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