Transcriptomics

Dataset Information

0

Bulk and single RNA sequencing on the role of Igf2bp2 in myeloid-biased HSC


ABSTRACT: Using RNA sequencing analysis on myeloid biased HSC, we compared gene expression profiles between WT and Igf2bp2 knock-out mice at young and old age. 1421 differentially expressed genes were identified in young myeloid biased HSC from Igf2bp2 knock-out mice compared to young WT; however, only 26 differentially expressed genes were identified in old myeloid biased HSC from Igf2bp2 knock-out mice compared to old WT. Compared to young WT myeloid biased HSC, myeloid biased HSC from young Igf2bp2 knock-out exhibits reduced expression of genes involved in metabolism and translation. In addition, single cell RNA sequencing analysis of myeloid biased HSC in young wildtype mice identified nine sub-clusters. HSCs from a sub-cluster with high expression of Igf2bp2 exhibit up-regulated IGF/PI3K/AKT signaling and increased expression of genes involved in HSC quiescence and maintenance. Altogether, the study provides a correlation and mechanism to understand the role of Igf2bp2 in HSC function and aging.

ORGANISM(S): Mus musculus

PROVIDER: GSE166176 | GEO | 2021/02/05

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2024-05-21 | PXD018535 | Pride
2019-01-16 | E-MTAB-7277 | ExpressionAtlas
2023-06-04 | E-MTAB-12890 | biostudies-arrayexpress
2022-01-21 | E-MTAB-11359 | biostudies-arrayexpress
2023-06-15 | E-MTAB-10083 | ExpressionAtlas
2011-11-11 | E-GEOD-32719 | biostudies-arrayexpress
2016-01-04 | E-MEXP-3309 | ExpressionAtlas
2011-05-06 | E-GEOD-27686 | biostudies-arrayexpress
| PRJNA699541 | ENA
2014-07-03 | E-MTAB-2439 | ExpressionAtlas