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Understanding the nitrate responsive regulatory modules in Rice

ABSTRACT: Understanding the nitrate responsive regulatory modules in Rice

PROVIDER: PRJNA701560 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13696500_1.fastq.gz	Fastqsanger.gz
	SRR13696500_2.fastq.gz	Fastqsanger.gz
	SRR13696501_1.fastq.gz	Fastqsanger.gz
	SRR13696501_2.fastq.gz	Fastqsanger.gz
	SRR13696502.fastq.gz	Fastqsanger.gz

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Understanding the nitrate responsive regulatory modules in Rice [RNA-seq]

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Understanding the nitrate responsive regulatory modules in Rice [MADS27OE, ChIP-seq]

Project description:Understanding the nitrate responsive regulatory modules in Rice [MADS27OE, ChIP-seq]

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Project description:Understanding the nitrate responsive regulatory modules in Rice [MADS27OE, RNA-seq]

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Understanding the nitrate responsive regulatory modules in Rice [RNA-seq]

Project description:To identify the differetially expressed genes under nitrate stress, RNA-seq was performed using total RNA extracted from root and seedling tissues from plants were grown in both low (0 mM) and high (100 mM) nitrate conditions. The analysis was performed in duplicates and the reads were mapped to rice (IRGSP-1) genome.

2022-04-01 | GSE166667 | GEO

Understanding the nitrate responsive regulatory modules in Rice [MADS27OE, RNA-seq]

Project description:MADS27 was differetially expressed during nitrate stress in roots of rice plant. In order to further verify the effect of MADS27 overexpression in rice root development, RNA-seq analysis was performed on plants overexpressing MADS27.

2022-04-01 | GSE166666 | GEO

Understanding the nitrate responsive regulatory modules in Rice [ncRNA-seq]

Project description:To identify the differetially expressed sRNAs under nitrate stress, sRNA-seq was performed using total RNA extracted from root and seedling tissues from plants were grown in both low (0 mM) and high (100 mM) nitrate conditions. The analysis was performed in duplicates and the reads were processed and analysed using UEA sRNA workbench.

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Understanding the nitrate responsive regulatory modules in Rice [MADS27OE, ChIP-seq]

Project description:To identify genome-wide binding sites of MADS27, we performed ChIP-seq experiment from 3xMyc-OsMADS27OE rice seedlings.

2022-04-01 | GSE166665 | GEO

Understanding the nitrate responsive regulatory modules in Rice

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-04-01 | GSE166669 | GEO

Effect of overexpression of a nitrate-inducible GARP-type transcriptional repressor NIGT1 in rice

Project description:Nitrogen is the most important macronutrient in plants and its supply induces responses in gene expression, metabolism and developmental processes. However, the molecular mechanisms underlying the nitrogen responses remain poorly understood. Here we show that the supply of nitrate but not ammonium immediately induces the expression of a transcriptional repressor gene in rice, designated NIGT1 (Nitrate-Inducible, GARP-type Transcriptional Repressor 1). The results of DNA-binding site selection experiments and electrophoretic mobility shift assays indicated that NIGT1 binds to DNA containing either of two consensus sequences, GAATC and GAATATTC. In transient reporter assays, NIGT1 was found to repress transcription from the promoters containing the identified NIGT1-binding sequences in vivo. Furthermore, NIGT1 repressed the activity of its own promoter, suggesting an auto-repression mechanism. Consistently, nitrate-induced NIGT1 expression was found to be downregulated after a transient peak during nitrate treatment, and the nitrate-induced expression of NIGT1 decreased in transgenic rice plants in which this gene was constitutively overexpressed. Furthermore, the chlorophyll content that could be a marker of nitrogen utilization was found to be decreased in NIGT1 overexpressors of rice grown with nitrate medium but not with ammonium medium. Thus, we propose NIGT1 as a nitrate-inducible and auto-repressible transcriptional repressor that may play a role in the nitrogen response in rice. Taken together with the fact that the NIGT1-binding sites are conserved in promoter sequences of Arabidopsis NIGT1 homologs, our findings imply the presence of a time-dependent complex system for nitrate-responsive transcriptional regulation that is conserved in both monocots and dicots.

2013-01-03 | GSE43243 | GEO

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