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Cotranscriptional RNA cleavage by Drosha homolog Pac1 triggers transcription termination in fission yeast.

ABSTRACT: Cotranscriptional RNA cleavage by Drosha homolog Pac1 triggers transcription termination in fission yeast.

PROVIDER: PRJNA702813 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR13737853.fastq.gz	Fastqsanger.gz
	SRR13737854.fastq.gz	Fastqsanger.gz
	SRR13737855.fastq.gz	Fastqsanger.gz
	SRR13737856.fastq.gz	Fastqsanger.gz
	SRR13737857.fastq.gz	Fastqsanger.gz

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Cotranscriptional RNA cleavage by Drosha homolog Pac1 triggers transcription termination in fission yeast. [RNA-Seq]

Project description:RNAse III is an evolutionarily conserved family of endoribonuclease that cleaves dsRNA structures. Here we studied RNAse III homolog Pac1 In fission yeast to shed light on underappreciated roles of Drosha. We found that Pac1 co-transcriptional cleavage of nascent hairpin RNA structures trigger transcriptional termination by creating an entry point for “torpedo” exonucleases – that is, exonuclease that degrade the nascent RNA until they bump into the transcribing polymerases. As such termination pathway decouple termination from pre-mRNA polyadenylation, the nascent transcript are destabilized upon Pac1 cleavage, therefore expending the paradigm of post-transcriptional regulation of gene expression.

2021-02-19 | GSE167041 | GEO

Cotranscriptional RNA cleavage by Drosha homolog Pac1 triggers transcription termination in fission yeast. [ChIP-Seq]

2021-02-19 | GSE167040 | GEO

Cotranscriptional RNA cleavage by Drosha homolog Pac1 triggers transcription termination in fission yeast. [ChIP-Seq]

Project description:Cotranscriptional RNA cleavage by Drosha homolog Pac1 triggers transcription termination in fission yeast. [ChIP-Seq]

| PRJNA702775 | ENA

Cotranscriptional RNA cleavage by Drosha homolog Pac1 triggers transcription termination in fission yeast. [RNA-Seq]

Project description:Cotranscriptional RNA cleavage by Drosha homolog Pac1 triggers transcription termination in fission yeast. [RNA-Seq]

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Cotranscriptional RNA cleavage by Drosha homolog Pac1 triggers transcription termination in fission yeast.

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-02-19 | GSE167065 | GEO

Co-transcriptional RNA cleavage by Drosha homolog Pac1 triggers transcription termination in fission yeast.

Project description:Transcription termination of protein-coding genes in eukaryotic cells usually relies on a tight coordination between the cleavage and polyadenylation of the pre-mRNA, and 5'-3' degradation of the downstream nascent transcript. Here we investigated the contribution of the essential fission yeast endonuclease Pac1, a homolog of human Drosha that cleaves hairpin RNA structures, in triggering polyadenylation-independent transcription termination. Using ChIP-sequencing in Pac1-deficient cells, we found that Pac1 triggers transcription termination at snRNA and snoRNA genes as well as at specific protein-coding genes. Notably, we found that Pac1-dependent premature termination occurred at two genes encoding conserved transmembrane transporters whose expression were strongly repressed by Pac1. Analysis by genome editing indicated that a stem-loop structure in the nascent transcript directs Pac1-mediated cleavage and that the regions upstream and downstream of the Pac1 cleavage site in the targeted mRNAs were stabilized by mutation of nuclear 3'-5' and 5'-3' exonucleases, respectively. Our findings unveil a premature transcription termination pathway that uncouples co-transcriptional RNA cleavage from polyadenylation, triggering rapid nuclear RNA degradation.

| S-EPMC8421224 | biostudies-literature

Pac1+/+ versus Pac1-/- TG-macrophages_LPS 6h

2006-01-10 | GSE3993 | GEO

Transcription profiling of mouse macrophaes and mast cells from Pac1+/+ versus Pac1-/- animals

Project description:This SuperSeries is composed of the following subset Series:; GSE3993: Pac1+/+ versus Pac1-/- TG-macrophages_LPS 6h; GSE3994: Pac1+/+ versus Pac1-/- BMMCs_IgE-DNP 2 h Experiment Overall Design: Refer to individual Series

2008-06-12 | E-GEOD-4014 | biostudies-arrayexpress

Transcription profiling of mouse Pac1+/+ versus Pac1-/- TG-macrophages treated with LPS - 6h

Project description:LPS-stimulated macrophages from Pac1+/+ and Pac1-/- mice were compared to assess gene expression changes in the absence of PAC-1 (also known as Dual specificity phosphatase 2). Experiment Overall Design: Age matched Pac1+/+ and Pac1-/- littermates were used to obtain equal numbers of thiolycollate-elicited macrophages. Pure populations of macrophages were stimulated with 100 ng/ml of LPS for 6 hours. 6 hours was chosen to assess mainly inflammatory gene expression.

2008-06-12 | E-GEOD-3993 | biostudies-arrayexpress

Pac1+/+ versus Pac1-/- in macrophages and mast cells

Project description:Pac1+/+ versus Pac1-/- in macrophages and mast cells

| PRJNA95111 | ENA