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Ovis aries strain:Subo mernio | breed:Mernio sheep

ABSTRACT: Comprehensive analysis of miRNAs during Merino sheep hair follicle development

PROVIDER: PRJNA705552 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14181045_1.fastq.gz	Fastqsanger.gz
	SRR14181045_2.fastq.gz	Fastqsanger.gz
	SRR14181046_1.fastq.gz	Fastqsanger.gz
	SRR14181046_2.fastq.gz	Fastqsanger.gz
	SRR14181047_1.fastq.gz	Fastqsanger.gz

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Project description:We report the application of High throughout sequencing to explore the differences of skin between Super Merino sheep(SM) and Small Tail Han sheep, which have remarkable phenotype differences on wool and hair follicle traits. We analysed the expression data by CLC genomic workbench 9.0 software. We find there are 435 differential expressional genes (DEGs) (127 were up-regulated and 308 were down-regulated) when STH sheep as control group. Some hair follicle KRTs, KAPs genes and hair follicle stem cells marker genes, were up-regulated in SM sheep. However, some of mammalian epidermal development complex (EDC) family genes were up-regulated in STH sheep. The GO and gene network analysis shown high expression genes in SM sheep enriched on type I interferon, lipid/fatty acid synthesis metabolism. This study provide more details in skin which control the development of follicle and wool in sheep.

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Project description:Here, we analyzed and identified the miRNA expression profile of three different intestinal tissues (i.e., duodenum, cecum, and colon) of sheep (Ovis aries) using high-throughput sequencing and bioinformatic methods. In total, 128 known miRNAs were identified, 526 novel miRNAs were predicted, and 202 differentially expressed miRNAs were found between the different tissues. Additionally, 4,422 candidate target genes were predicted, and 185 non-redundant GO annotation terms were identified using enrichment analysis. A total of 529 target genes were found to participate in 37 KEGG biological pathways, and 270 of these genes were significantly enriched in the metabolism category.

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