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Anti-PDGFcc antibody treatment increases adipogenesis pathways in inguinal white adipose tissue

ABSTRACT: Anti-PDGFcc antibody treatment increases adipogenesis pathways in inguinal white adipose tissue

PROVIDER: PRJNA715758 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14009562_1.fastq.gz	Fastqsanger.gz
	SRR14009562_2.fastq.gz	Fastqsanger.gz
	SRR14009563_1.fastq.gz	Fastqsanger.gz
	SRR14009563_2.fastq.gz	Fastqsanger.gz
	SRR14009564_1.fastq.gz	Fastqsanger.gz

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Anti-PDGFcc antibody treatment increases adipogenesis pathways in inguinal white adipose tissue

Project description:Purpose: The goals of this experiment were to examine the transcriptional profiles (RNA-seq) of inguinal white adipose tissue (iWAT) in mice subjected to the indicated diets and treatments for eight weeks. Methods: 6-week-old C57BL6/J mice fed a control or a high-fat diet were treated with anti-PDGFcc neutralizing antibodies or Goat IgG for 2 weeks of treatment. Antibodies were administered by i.p. every two days. Four animals were treated in each condition. The resulting iWAT from these mice were sequenced in duplicates, aligned with STAR, quantified with featureCounts, and then differential gene expression analyses were performed using limma. Results: An unbiased analysis of hallmark pathways in iWAT of anti-PDGFcc antibody-treated mice and control animals using a pre-ranked Gene Set Enrichment Analysis (GSEA) identified adipogenesis as the pathway most differentially regulated by PDGFcc blockade when accounting for diet effects. By contrast, the hallmark fatty acid metabolism pathway which includes genes involved in lipid uptake, lipid oxidation, and lipid mobilization was not significantly enriched after anti-PDGFcc treatment. Similarly, hallmark pathways glycolysis, MTORC1 signaling, or TGFb signaling were not differentially regulated by anti-PDGFcc treatment after accounting for diet effects. An additional GSEA analysis of genes involved in lipid synthesis and storage identified a subset of genes up-regulated by PDGFcc blockade regardless of diet, which included several negative regulators of lipid synthesis and storage such as Insig1, Ppara, and Idh2. Conclusions: A GSEA identified adipogenesis as the pathway most differentially regulated by PDGFcc blockade when accounting for diet effects. Key genes involved included several negative regulators of lipid synthesis and storage such as Insig1, Ppara, and Idh2.

2021-03-20 | GSE169226 | GEO

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Project description:Excessive fat accumulation is a major risk factor for the development of type 2 diabetes.To determine the mechanisms by wich TP53INP2 regulates adipogenesis, gene expression profile was performed in inguinal white adipose tissue fromTP53INP2-deficient mice.

2018-04-03 | GSE103586 | GEO

Mouse interscapular brown, subscapular white and inguinal white adipose tissue

Project description:Adult mouse were kept at room temperature, RNAseq was performed for interscapular brown, subscapular white and inguinal white adipose tissue

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Project description:We use microarray to analyze gene expression after adipocyte constitutively active HDAC4 expression in inguinal white adipose tissue

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Repression of Cebpa-L1-AD-En2 enhancer decreases Cebpa expression in inguinal white adipose tissue and affects adipose tissue development

Project description:To investigate whether Cebpa-L1-AD-En2 produces a marked effect on adipogenesis in mouse adipose tissue, we injected the dCas9-KARB-En2 lentiviral system (iWAT_Cebpa-En2 group) or dCas9-KRAB-GFP (Control group) directly into mouse inguinal white adipose tissue (iWAT). We conducted RNA-seq analysis on the iWAT of iWAT-En2 group mice and control group mice at 13 weeks of age

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Expression analysis of inguinal white adipose tissue

Project description:Expression analysis of inguinal white adipose tissue

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Biological Aging and Circadian Mechanisms in Murine Brown Adipose Tissue, Inguinal White Adipose Tissue, and Liver

Project description:This SuperSeries is composed of the following subset Series: GSE25323: Biological Aging and Circadian Mechanisms in Murine Brown Adipose Tissue, Inguinal White Adipose Tissue, and Liver (Nov 2009 dataset) GSE25324: Biological Aging and Circadian Mechanisms in Murine Brown Adipose Tissue, Inguinal White Adipose Tissue, and Liver (Jan 2010 dataset) Refer to individual Series

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White adipose tissue resident macrophages are required for adipogenesis and energy storage.

Project description:White adipose tissue (WAT) stores energy in the form of lipids. WAT macrophages cause adipose tissue inflammation and insulin resistance when intake exceeds expenditures, but their contribution to homeostasis remain poorly understood. Here, we show that two populations, ã and ä, of TNF-producing bone marrow-derived monocytic cells are transiently recruited to damaged adipocytes and mediate insulin resistance. In contrast, we identify α/β yolk-sac derived resident macrophages that produce adipogenic growth factors, and form a niche required for adipogenesis as white adipocytes do not differentiate in their absence. Production of adipogenic growth factors by α/β macrophages increases with lipid storage, but they do not contribute to inflammation. Thus the molecular identities and functions of resident macrophages and BM-derived cells are specified by their lineage rather than diet variation. These results identify an essential function of WAT resident macrophages in adipogenesis and energy homeostasis, separate from inflammation mediated by the recruitment of inflammatory leukocytes.

2020-10-24 | GSE106084 | GEO

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