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New insights into the regulatory mechanism of m6A mRNA methylation on muscle stem cells

ABSTRACT: New insights into the regulatory mechanism of m6A mRNA methylation on muscle stem cells

PROVIDER: PRJNA716604 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14044845_1.fastq.gz	Fastqsanger.gz
	SRR14044845_2.fastq.gz	Fastqsanger.gz
	SRR14044846_1.fastq.gz	Fastqsanger.gz
	SRR14044846_2.fastq.gz	Fastqsanger.gz
	SRR14044847_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

New insights into the regulatory mechanism of m6A mRNA methylation on muscle stem cells

Project description:In the conditional knockout mouse model, explore the role of m6A modification in the process of muscle damage repair.The molecular regulation mechanism of m6A modification on muscle regeneration was found through the study of related signaling pathways.

2021-05-26 | GSE169432 | GEO

Hakai is required for stabilization of core components of the m6A mRNA methylation machinery

Project description:N6-methyladenosine (m6A) is the most abundant internal modification on mRNA which influences most steps of mRNA metabolism and is involved in several biological functions. The E3 ubiquitin ligase Hakai was previously found in complex with components of the m6A methylation machinery in plants and mammalian cells but its precise function remained to be investigated. Here we show that Hakai is a conserved component of the methyltransferase complex in Drosophila and human cells. In Drosophila, its depletion results in reduced m6A levels and altered m6A-dependent functions including sex determination. We show that its ubiquitination domain is required for dimerisation and interaction with other members of the m6A machinery, while its catalytic activity is dispensable. Finally, we demonstrate that the loss of Hakai destabilizes several subunits of the methyltransferase complex, resulting in impaired m6A deposition. Our work adds new functional and molecular insights into the mechanism of the m6A mRNA writer complex.

2021-04-29 | PXD022294 | Pride

Structural insights into regulatory mechanism of RNA modification by human m6A-METTL associated complex

Project description:N6-methyladenosine (m6A) is the most abundant ribonucleotide modification among the eukaryotic messenger RNAs (mRNAs). The m6A “writer” is composed of an m6A-METTL complex (MAC), the catalytic subunit, and an m6A-METTL associated complex (MACOM), the regulatory subunit essential for the enzymatic activity. Here we report the cryo-electron microscopy (cryo-EM) structures of MACOM at 3.0-Å resolution, uncovering that WTAP and VIRMA form the core structure of MACOM and ZC3H13 stretches the conformation by binding VIRMA. The lower 4.4-Å resolution cryo-EM datamap of MACOM in complex with MAC, in combination with crosslinking mass spectrometry and GST-pulldown analysis, elucidates a plausible model of the m6A writer complex, in which MACOM binds MAC mainly through WTAP and METTL3 interaction and both components directly contact with RNA substrates revealed by 4-thiouridine-labeled RNA crosslinking analysis. This work establishes the possible assembly mechanism of MACOM and MAC as an active m6A writer for RNA substrate recognition and modification.

2022-06-23 | PXD028456 |

Widespread Occurrence of m6A in bacterial mRNA

Project description:N6-methyladenosine (m6A) is one of the most abundant modifications in eukaryotic RNA. Recent mapping of m6A methylomes in mammals, yeast, and plants as well as characterization of m6A methyltransferases, demethylases, and binding proteins have revealed regulatory functions of this dynamic RNA modification. In bacteria, although m6A is present in ribosomal RNA (rRNA), its occurrence in messenger RNA (mRNA) still remains elusive. Here, we used liquid chromatography-mass spectrometry (LC-MS) to calculate the m6A/A ratio in mRNA from a wide range of bacterial species, which demonstrates that m6A is an abundant mRNA modification in tested bacteria. Subsequent transcriptome-wide m6A profiling in Escherichia coli and Pseudomonas aeruginosa revealed a conserved distinct m6A pattern that is significantly different from that in eukaryotes. Most m6A peaks are located inside open reading frames (ORF), and carry a unique consensus motif (GCCAU). Functional enrichment analysis of bacterial m6A peaks indicates that the majority of m6A-modified transcripts are associated with respiration, amino acids metabolism, stress response, and small RNAs genes, suggesting potential regulatory roles of m6A in these pathways. m6A profiling in E.coli and P.aeruginosa mRNA

2015-07-08 | E-GEOD-65347 | biostudies-arrayexpress

Profiling of mRNA N6-Adenosine Methylation (m6A) in Arabidopsis

Project description:m6A profiling in two accessions of Arabidopsis thaliana (Can-0 and Hen-16) using the m6A-targeted antibody coupled with high-throughput sequencing m6A-seq in two accessions of Arabidopsis, two replicates for each sample

2014-11-06 | E-GEOD-59154 | biostudies-arrayexpress

m6A modification regulates lung fibroblast-to-myofibroblast transition through modulating KCNH6 mRNA translation

Project description:In summary, we characterized the role of m6A modification in pulmonary fibrosis. We reveal that m6A modification is increased in bleomycin induced pulmonary fibrosis mice model, FMT-derived myofibroblasts and idiopathic pulmonary fibrosis patient lung samples. Lowering m6A level through silencing METTL3 suppress FMT process in vitro and vivo. Fundamentally, m6A modification regulates FMT by modulating the translation of KCNH6 mRNA in a YTHDF1 dependent manner. This study provides novel insights into the mechanism of FMT process and suggests m6A modification intervention may be a promising therapeutic strategy for pulmonary fibrosis.

2021-06-02 | GSE164151 | GEO

N6-methyl adenosine (m6A) recruits and repels proteins to regulate mRNA homeostasis

Project description:RNA modifications are integral to regulation of RNA metabolism. One such abundant mRNA modification is m6A, which impacts various aspects of RNA metabolism including splicing, transport and degradation. Current knowledge about proteins recruited to m6A to carry out these molecular processes is still limited. Here we describe a comprehensive and systematic mass spectrometry-based screening of m6A interactors in various cell types and species. Amongst the main findings, we identified G3BP1 as a protein, which is repelled by m6A and which positively regulates mRNA stability in an m6A regulated manner. Furthermore, we identified FMR1 as a novel, RNA sequence context dependent m6A reader, thus revealing a connection between an mRNA modification and an autism spectrum disorder. Collectively, our data represents a rich resource for the community and sheds further light on the complex interplay between m6A, m6A interactors and mRNA homeostasis.

2017-08-22 | PXD006498 | Pride

Dynamic m6A mRNA methylation reveals novel insights into mechanisms of chemical carcinogenesis

Project description:m6A epitranscriptome profiling in four different chemical carcinogen induced transformation models uncovered the dynamic changes of m6A modifications during chemical carcinogenesis.

2018-12-01 | GSE112970 | GEO

Dynamic m6A mRNA methylation reveals novel insights into mechanisms of chemical carcinogenesis

Project description:Dynamic m6A mRNA methylation reveals novel insights into mechanisms of chemical carcinogenesis

| PRJNA449645 | ENA

m6A in 5â untranslated regions promotes cap-independent translation of mRNA

Project description:N6-methyladenosine (m6A) is a widespread internal RNA modification whose function is poorly understood. Here we report that m6A residues within the 5'UTR promote a novel form of cap-independent translation which is mediated through an interaction between m6A residues and the translation initiation factor, eIF3. We present eIF3a PAR-iCLIP data which demonstrate that eIF3 predominantly binds mRNAs within the 5'UTR. eIF3 binding sites are also in proximity to m6A residues within the 5'UTR of cellular mRNAs. Two replicates of eIF3a PAR-iCLIP in HEK293T cells.

2015-10-26 | E-GEOD-73405 | biostudies-arrayexpress

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