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Arabidopsis thaliana KO1 and KO3 deletion lines of the selective autophagy receptor AtNBR1 - roots and shoots

ABSTRACT: Arabidopsis thaliana KO1 and KO3 deletion lines of the selective autophagy receptor AtNBR1 - roots and shoots

PROVIDER: PRJNA716658 | ENA |

REPOSITORIES: ENA

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Arabidopsis thaliana KO1 and KO3 deletion lines of the selective autophagy receptor AtNBR1 - roots and shoots

Project description:We used the CRISPR/Cas9 technique to construct nbr1-KO lines (KO1 and KO3) in order to test the effects of AtNBR1 depletion. Reduced expression of several ABA-up regulated genes were observed in shoots of the two KO lines. Grant number: 2014/15/B/NZ3/04854 Grant funding source: National Science Centre (Poland) Grantee Name: Agnieszka Sirko Grant title: Upstream regulators and cellular targets of the selective autophagy cargo receptor AtNBR1 in different phases of sulfur deficiency in plants.

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Arabidopsis thaliana KO1 and KO3 deletion lines of the selective autophagy receptor AtNBR1

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Arabidopsis thaliana KO1 and KO3 deletion lines of the selective autophagy receptor AtNBR1

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Overexpression of the selective autophagy receptor AtNBR1

Project description:Cargo receptors are well established elements of the selective autophagy in all eukaryotes. The NBR1-like family of such receptors is evolutionarily conserved, however plants have only one member of the family, which is a functional and structural hybrid of its two animal counterparts, p62 (SQSTM1) and NBR1. Examination of plants overexpressing NBR1 indicates that they are oversensitive to TOR inhibitors and have more autophagosomes than the parental lines. The observed changes in shoots suggested induction of ABA signalling pathway, what was further corroborated by the germination tests and the assay of ABA level. The transcriptome changes in roots, such as lower level of snoRNA and some tRNAs, suggested inhibition of ribosome biogenesis, what is supported by identification of RPS6, an important component of TOR-dependent translational control, as a novel partner of NBR1. Grant number: 2014/15/B/NZ3/04854 Grant funding source: National Science Centre (Poland) Grantee Name: Agnieszka Sirko Grant title: Upstream regulators and cellular targets of the selective autophagy cargo receptor AtNBR1 in different phases of sulfur deficiency in plants.

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