Project description:We seprated the primodial germ cells using MACS from E12.5 stages in WT and Rif1-knock out mice, and using modified Smart-seq2 methods to get 1,000 cells library.
Project description:High-throughout RNA microarray results revealed different gene expression, including arg1, VEGF, TGF-beta, and IL-10, in ischemic myocardium of endothelial S1pr1 knock-out mice, compared to WT mice.
Project description:Endothelial cells (ECs) serve as a semipermeable barrier and play a key role in acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). It is well known that fibroblast growth factor receptor 1 (FGFR1) is a requirement for maintaining vascular integrity. However, how endothelial FGFR1 exerts its function remains obscure in ALI/ARDS. Here, we performed RNA-seq analysis to investigate transcriptional changes of ECs between saline and LPS group, WT (Fgfr1loxp/loxp) and endothelial Fgfr1-conditional knock out mice (Fgfr1iΔEC/iΔEC mice).
Project description:We used microarrays to detail the global programme gene expression of Phf8 knock out and wild type mice Different expression profile were compared between Phf8 knock out and wild type mice
Project description:To investigate the functional importance of a nucleoside transporter, the mENT1 (Slc29a1) was knocked out in mice. The gene expression profile was compared between wildtype and mENT1 knock out mice in two tissues.
Project description:Affymetrix microarray analysis of molecular changes after myocardial infarction. Samples of heart tissue were analyzed after myocardial infarction from WT and reg3beta knock-out mice. Samples from scar tissue and samples adjacent to the scar were analyzed. In the experiment we primarily compared infarction zone of wild-type to infarction zone of knock-out animals, and remote zone of wild-type to remote zone of knock-outs.
Project description:Ewing’s Sacroma (EWS) is a carcinogenic bone tumor which is predominantly occurred in young adults. EWS RNA binding protein 1 (Ewsr1) protein is well-known as a multifunctional protein and its epigenetic signatures contribute to the pathogenesis of various human disease such as neurodegenerative disorders, skin development, or tumorigenic process. However, the specific cellular function and physiological characteristics of Ewsr1 are still ambiguous. In this study, therefore, we applied TMT-labeled quantitative mass spectrometry-based proteomic approach to investigate the global proteome changes of brain tissue between Ewsr1 knock-out (KO) and wild type (WT) mice (n=3, each).